The green impact: bacterioplankton response toward a phytoplankton spring bloom in the southern North Sea assessed by comparative metagenomic and metatranscriptomic approaches

Frontiers in Microbiology
Bernd WemheuerRolf Daniel

Abstract

Phytoplankton blooms exhibit a severe impact on bacterioplankton communities as they change nutrient availabilities and other environmental factors. In the current study, the response of a bacterioplankton community to a Phaeocystis globosa spring bloom was investigated in the southern North Sea. For this purpose, water samples were taken inside and reference samples outside of an algal spring bloom. Structural changes of the bacterioplankton community were assessed by amplicon-based analysis of 16S rRNA genes and transcripts generated from environmental DNA and RNA, respectively. Several marine groups responded to bloom presence. The abundance of the Roseobacter RCA cluster and the SAR92 clade significantly increased in bloom presence in the total and active fraction of the bacterial community. Functional changes were investigated by direct sequencing of environmental DNA and mRNA. The corresponding datasets comprised more than 500 million sequences across all samples. Metatranscriptomic data sets were mapped on representative genomes of abundant marine groups present in the samples and on assembled metagenomic and metatranscriptomic datasets. Differences in gene expression profiles between non-bloom and bloom samples were rec...Continue Reading

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Citations

Aug 31, 2016·Nature Microbiology·Sara BillerbeckMeinhard Simon
Feb 1, 2017·Environmental Microbiology·Ying SunHaiwei Luo
Jul 22, 2017·The ISME Journal·Theoden Vigil-StenmanMartin Ekman
Jan 31, 2016·Applied and Environmental Microbiology·Yao ZhangHaiwei Luo
Feb 19, 2019·Microbes and Environments·Takafumi KataokaDaiki Nomura
Nov 20, 2018·Frontiers in Microbiology·Marwan E MajzoubSuhelen Egan
Jun 19, 2018·Frontiers in Microbiology·Rolf DanielBernd Wemheuer
Jun 3, 2021·Microorganisms·Kathryn ForconeCynthia B Silveira
Jun 20, 2021·The ISME Journal·Xiaoyuan FengHaiwei Luo
Aug 19, 2021·Environmental Microbiome·Ben FrancisRudolf Amann

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Datasets Mentioned

BETA
SRA061816

Methods Mentioned

BETA
Fluorescence
PCR
Illumina sequencing

Software Mentioned

Trimmomatic
Metavelvet
QIIME
BLAST
Ocean Data View
Velvet
Usearch
Bowtie
UCLUST
UProC

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