PMID: 11309793Apr 20, 2001Paper

The growth of chondrocytes into a fibronectin-coated biodegradable scaffold

Journal of Biomedical Materials Research
R S BhatiS W Shalaby

Abstract

Porous scaffolds made from a biodegradable copolymer of trimethylene carbonate and glycolide were evaluated for tissue-engineered medical products. We examined the scaffold coated with cell adhesion protein and fibronectin and cultured under a dynamic mixing condition to enhance the growth of chondrocytes. Our hypothesis was that the combination of coating and dynamic mixing would be beneficial to the viability of the chondrocytic cells. Fibronectin was selected as the model protein because of its availability and routine assaying methods. Sterile samples of scaffolds of about 1 mm in thickness were coated with fibronectin at 37 degrees C for 1.5 h. Four groups of scaffolds were used: uncoated static or dynamic, and coated static or dynamic. Scaffold samples were placed in either a Petri dish or a spinner flask (static vs. dynamic groups) after inoculation with rat chondrocytes of an initial cell density of 1.29 x 10(5) cell/mL. After 7, 14, 21, and 28 days, each sample was fixed, embedded, and sectioned at 5 micro thickness. The sections were double-label immunostained using antibodies against cellular fibronectin synthesized by adherent cells as a measure of cell viability. A Hoechst 33258 nuclear stain was used to measure th...Continue Reading

References

Jul 1, 1990·The Journal of Bone and Joint Surgery. British Volume·O BöstmanP Rokkanen
Oct 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K J McCarthy, J R Couchman
Jul 1, 1989·Journal of the American Academy of Dermatology·T J Ryan
Aug 15, 1995·Biochemical and Biophysical Research Communications·M A AttawiaC T Laurencin
Apr 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K J McCarthyJ R Couchman
Feb 1, 1994·International Journal of Oral and Maxillofacial Surgery·W C PuelacherC A Vacanti
Mar 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K J McCarthyJ R Couchman
Nov 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E N LammeE Middelkoop
Feb 1, 1996·Journal of Biomedical Materials Research·K Kottke-MarchantR E Marchant
Jul 1, 1997·Journal of Biomedical Materials Research·S L IshaugA G Mikos
Jun 1, 1997·Experimental Dermatology·J B PetriK Herrmann
Jun 1, 1997·Cell Structure and Function·M ShimizuJ Koga
Aug 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S GronthosP J Simmons
Sep 7, 2001·Scientific American·D E Ingber

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Citations

Feb 8, 2006·Journal of Mathematical Biology·Greg LemonKevin M Shakesheff
Jun 13, 2009·Journal of Materials Science. Materials in Medicine·Chunli YangChunyu Yang
Mar 11, 2003·Tissue Engineering·Eric M Darling, Kyriacos A Athanasiou
Mar 31, 2009·Tissue Engineering. Part C, Methods·Ashwin NairLiping Tang
Aug 25, 2005·IEEE Transactions on Nanobioscience·Mengyan LiMichael J Mcshane
Sep 1, 2005·Journal of Biomaterials Science. Polymer Edition·Sang Ho ChoJin Ho Lee
Sep 14, 2007·Expert Review of Medical Devices·Andrew S RowlandsJustin J Cooper-White
Apr 14, 2006·Expert Opinion on Biological Therapy·Ying Yang, Alicia J El Haj
May 8, 2007·Advanced Drug Delivery Reviews·Hyun Jung Chung, Tae Gwan Park
Nov 5, 2008·Journal of Biomedical Materials Research. Part a·Yung-Chih Kuo, Ya-Ru Hsu
Jun 12, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Debi P MukherjeeByron K Hayes
Mar 26, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Jun XieTakehisa Matsuda
Jun 13, 2017·Tissue Engineering. Part C, Methods·Naser NasrollahzadehDominique P Pioletti
Jan 1, 2012·Journal of Functional Biomaterials·Zohreh IzadifarWilliam Kulyk
Aug 18, 2016·Organic & Biomolecular Chemistry·Jing HuJian Yin
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Sibylle GradMauro Alini
May 22, 2007·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Federico Caneva SoumetzCarmelina Ruggiero
Dec 20, 2008·Journal of Biomedical Materials Research. Part a·I-Chi LeeTai-Horng Young
Oct 1, 2010·Advanced Materials·Prakriti Tayalia, David J Mooney
Jun 5, 2019·Polymers·Silviya BoyadzhievaKlaus Kruttwig
Aug 22, 2018·Drug and Chemical Toxicology·Zeynep Köksal, Zuhal Alim
Jan 8, 2021·Annals of Biomedical Engineering·Anamarija RoginaHrvoje Ivanković
Nov 11, 2003·Biomacromolecules·Ann-Christine Albertsson, Indra K Varma

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