PMID: 7020428Aug 1, 1981Paper

The growth of primary glomerular cells as a confluent monolayer in a chemically defined serum-free medium: specific requirements for prostaglandins and extracellular matrix molecules

The American Journal of Pathology
T D OberleyJ V Muth

Abstract

Glomerular cells grown in a defined medium consisting of Waymouth's MB 752/1 plus insulin, transferrin, sodium pyruvate, and nonessential amino acids were shown to require prostaglandin E1 and either fibronectin, laminin, of heparan sulfate to form a confluent monolayer. Under these conditions cells did not grow on type IV collagen. Dibutyryladenosine-3,5-cyclic monophosphoric acid decreased the time needed for the cells to reach confluency. The results suggest a requirement for extracellular matrix molecules for growth of glomerular cells in defined medium, probably a requirement of adhesive molecules for cell attachment. The role of prostaglandins is less clear, but prostaglandin appears to be necessary for cell division. Since the cells growing in defined medium containing prostaglandin E1 and extracellular matrix molecules are epithelial in morphology, we suggest they may represent the stem cell precursors of either glomerular visceral or parietal epithelial cells, or both. The growth of glomerular cells in medium containing prostaglandins and extracellular matrix molecules is completely inhibited by sodium butyrate. Immunofluorescent studies show that the majority of cells show only faint staining for fibronectin along per...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.