PMID: 6992059Feb 1, 1980Paper

The growth of Trypanosoma cruzi in human diploid cells for the production of trypomastigotes

Parasitology
C J SandersonC E Twomey

Abstract

The use of human diploid cell lines of finite life for the in vitro production of Trypanosoma cruzi is described. Both MRC5 and WI38 cells release trypomastigotes with less than 5% amastigotes. This could form the basis for biochemical and immunological studies, which were previously limited by the problems of separating parasites from blood. By selecting the in vitro passage number of the parasite it is possible to select for either the broad or the slender forms of trypomastigotes, allowing comparative studies of these forms within a single strain of the parasite. It is also possible to isolate amastigotes by disrupting the cells before trypomastigotes appear, and separating them from cell debris with Metrizamide. It is shown that by incorporating [3H]uridine in the cell-culture medium, labelled trypomastigotes are obtained. The release of this label (putative RNA) provides a relatively simple isotopic assay for parasite death. Examples of this assay for testing drug toxicity and in immunological lysis are presented.

References

Dec 1, 1978·International Journal for Parasitology·A F LópezC J Sanderson
Jul 1, 1975·The Journal of Experimental Medicine·N NogueiraZ Cohn
Jul 11, 1970·Nature·J P JacobsJ P Baille
Dec 1, 1972·Annals of Tropical Medicine and Parasitology·T A MariaZ Brener
Feb 1, 1970·Experimental Parasitology·E Rodriguez, C J Marinkelle
Jul 1, 1948·Parasitology·H MEYER, M XAVIER DE OLIVEIRA

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Citations

Jan 1, 1987·Transactions of the Royal Society of Tropical Medicine and Hygiene·A R Bogliolo, D G Godfrey
Apr 1, 1984·Molecular and Biochemical Parasitology·D E Lanar, J E Manning
Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·E TetaudT Baltz
Jul 1, 1984·The Journal of Cell Biology·G T Williams
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·A M da SilvaI Mota
Jan 1, 1985·Zeitschrift Für Parasitenkunde·F GamarroL M Ruiz-Pérez
Jan 1, 1983·Zeitschrift Für Parasitenkunde·T U de Carvalho, W de Souza
Feb 1, 1985·Experimental Parasitology·T U De CarvalhoW De Souza
Jun 1, 1992·Applied Biochemistry and Biotechnology·Y M GomesL Monjour
Aug 2, 2014·Parasite : Journal De La Société Française De Parasitologie·Gabriel Alberto Duran-RehbeinJohn Mario Gonzalez
Sep 1, 1987·The American Journal of Tropical Medicine and Hygiene·V R Scott, T R Matthews
Aug 1, 1986·Infection and Immunity·R A WrightsmanJ E Manning
Oct 1, 1990·Infection and Immunity·W Wagner, M So

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