The Growth Rate, Immune Status, Duodenal Development, and Cecal Microbial Diversity of 24-Day-Old Offspring of SD Rats Received Bacillus subtilis-Cu or CuSO4 During Pregnancy and Lactation Periods

Biological Trace Element Research
Guodong LiuMin Kong

Abstract

A total of 108 pregnant and lactation SD rats were divided into six groups, daily and orally dosed with Bacillus subtilis-copper (CuBs) and CuSO4 (CuS) both at three doses equivalent to 1.5 mg (Cu deficiency), 3 mg, and 6 mg Cu per kg diet, and the effects of the Cu source and dosage on the growth rate, immune status, duodenal development, and cecal microbial diversity were examined on 24-day-old offspring rats. The six offspring rats from each group were randomly selected for measuring the body weight gain and taking blood samples, and three rats were sacrificed for taking duodenum and cecum content samples. We found CuBs increased the body weight gain, development of duodenal villi, and survival rate of the offspring; increased the IgM content and lysozyme activity in serum; reduced the intestinal permeability; and increased the abundances of Lachnospiraceae, Ruminococcaceae, and Intestinibacter in the cecal content, when compared with CuS. We also found that Cu deficiency showed detrimental effects on the body weight gain and length, the survival rate of the offspring, and the immune indices in serum, as well as the increased intestinal permeability. We concluded that CuBs is better Cu source than CuSO4 for reproductive rats.

References

Dec 24, 1997·Genomics·W R PearsonW Miller
Jan 8, 2000·The Surgical Clinics of North America·R P Rabinowitz, E S Caplan
Apr 25, 2003·Poultry Science·J A Patterson, K M Burkholder
Jun 25, 2003·Toxicology·Lisa M Gaetke, Ching Kuang Chow
Nov 4, 2005·The New England Journal of Medicine·Johannes HäberleHans G Koch
May 16, 2006·Journal of Applied Microbiology·S ParvezH-Y Kim
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oliver PabstReinhold Förster
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Feb 19, 2008·Nature Chemical Biology·Byung-Eun KimDennis J Thiele
Jul 16, 2008·Inflammatory Bowel Diseases·S C NgS C Knight
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Sep 10, 2011·Bioinformatics·Tanja Magoč, Steven L Salzberg
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Dec 3, 2014·Nature Reviews. Gastroenterology & Hepatology·Lena ÖhmanMagnus Simrén
Dec 23, 2015·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Márcia H FukugaitiMario J Avila-Campos
Mar 24, 2016·World Journal of Gastroenterology : WJG·Yu-Yuan LiYu-Qiang Nie

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Citations

Sep 2, 2019·Biological Trace Element Research·Yanping HuangMin Kong

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Methods Mentioned

BETA
Scanning Electron
scanning electron microscopy
PCR
electrophoresis

Software Mentioned

QIIME
LEfSe
FASTX
FLASH
UCHIME
GenStat
mothur
Trimmomatic
- Toolkit

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