The GTPase Ran regulates chromosome positioning and nuclear envelope assembly in vivo

Current Biology : CB
Chisa BambaEisuke Nishida

Abstract

The GTPase Ran is known to regulate transport of proteins across the nuclear envelope. Recently, Ran has been shown to promote microtubule polymerization and spindle assembly around chromatin in Xenopus mitotic extracts and to stimulate nuclear envelope assembly in Xenopus or HeLa cell extracts. However, these in vitro findings have not been tested in living cells and do not necessarily describe the generalized model of Ran functions. Here we present several lines of evidence that Ran is indispensable for correct chromosome positioning and nuclear envelope assembly in C. elegans. Embryos deprived of Ran by RNAi showed metaphase chromosome misalignment and aberrant chromosome segregation, while astral microtubules seemed unaffected. Depletion of RCC1 or RanGAP by RNAi resulted in essentially the same defects. The immunofluorescent staining showed that Ran localizes to kinetochore regions of metaphase and anaphase chromosomes, suggesting the role of Ran in linking chromosomes to kinetochore microtubules. Ran was shown to localize to the nuclear envelope at telophase and during interphase in early embryos, and the depletion of Ran resulted in failure of nuclear envelope assembly. Thus, Ran is crucially involved in chromosome posit...Continue Reading

References

Jan 1, 1993·The Journal of Cell Biology·M RenM G Rush
Oct 6, 1998·Annual Review of Biochemistry·I W Mattaj, L Englmeier
Nov 6, 1998·Nature·L Timmons, A Fire
Dec 16, 1998·Science·UNKNOWN C. elegans Sequencing Consortium
May 14, 1999·Current Biology : CB·P KalabM Dasso
Dec 28, 1999·Annual Review of Cell and Developmental Biology·D Görlich, U Kutay
Jul 27, 2000·Molecular Cell·M HetzerI W Mattaj
Jun 13, 2001·The Journal of Cell Biology·K OegemaA A Hyman

❮ Previous
Next ❯

Citations

Mar 27, 2010·Cellular and Molecular Life Sciences : CMLS·Thomas Müller-ReichertMartin Srayko
May 29, 2007·Plant Cell Reports·Lian MaZhongming Zhang
Jun 6, 2003·Current Opinion in Cell Biology·B Booth Quimby, Mary Dasso
Dec 20, 2002·Journal of Structural Biology·Merav CohenYosef Gruenbaum
May 15, 2008·Nature Reviews. Molecular Cell Biology·Paul R Clarke, Chuanmao Zhang
Aug 26, 2003·Molecular Biology of the Cell·Christoph SegbertOlaf Bossinger
Sep 19, 2003·Molecular Biology of the Cell·Stephanie C Ems-McClungClaire E Walczak
Jun 30, 2006·Molecular Biology of the Cell·Arturo V OrjaloDouglass J Forbes
Mar 9, 2012·Molecular Biology of the Cell·Hanako HayashiAkatsuki Kimura
Feb 2, 2011·Journal of Experimental Botany·Thushani Rodrigo-PeirisIris Meier
Sep 21, 2004·Annual Review of Plant Biology·Eric LamKoichi Watanabe
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Yixian Zheng
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Martin W HetzerIain W Mattaj
Sep 27, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Yun-Kao CaoQing-Yuan Sun
Jan 31, 2007·The Journal of Cell Biology·Julien DumontMarie-Hélène Verlhac
Sep 29, 2004·The Journal of Cell Biology·Oliver J Gruss, Isabelle Vernos
Mar 26, 2003·The Journal of Cell Biology·Kathryn J RyanSusan R Wente
Jul 12, 2011·Gene·Zhe ZhaoXiaobo Zhang
Jul 7, 2009·Seminars in Cell & Developmental Biology·Stephen A Adam
Dec 5, 2015·Frontiers in Cell and Developmental Biology·Jack W C ChenJames G Wakefield
Jul 23, 2008·Molecular Reproduction and Development·Namdori R MtangoKeith E Latham
Jul 30, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Christian Zierhut, Hironori Funabiki
Apr 10, 2003·Biochemical and Biophysical Research Communications·Ward LutzRajiv Kumar
Feb 14, 2012·Biochimica Et Biophysica Acta·Yoichi MiyamotoKate L Loveland
Dec 19, 2003·Current Biology : CB·Richard Wozniak, Paul R Clarke
Aug 15, 2002·Current Biology : CB·Mary Dasso
Apr 25, 2006·Current Biology : CB·Maria D KoffaIain W Mattaj
Jul 18, 2002·Current Biology : CB·Nadia Trieselmann, Andrew Wilde
Dec 6, 2005·Developmental Cell·Karim NacerddineAnne Dejean
Nov 5, 2004·Molecular Cell·Amnon Harel, Douglass J Forbes
Apr 30, 2008·Biochimica Et Biophysica Acta·Ouadah HadjebiJose Luis Rosa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.