PMID: 9531539May 9, 1998Paper

The guanosine nucleotide (p)ppGpp initiates development and A-factor production in myxococcus xanthus

Genes & Development
B Z HarrisMitchell Singer

Abstract

Guanosine 3'-di-5'-(tri)di-phosphate nucleotides [(p)ppGpp], synthesized in response to amino acid limitation, induce early gene expression leading to multicellular fruiting body formation in Myxococcus xanthus. A mutant (DK527) that fails to accumulate (p)ppGpp in response to starvation was found to be blocked in development prior to aggregation. By use of a series of developmentally regulated Tn5lac transcriptional fusion reporters, the time of developmental arrest in DK527 was narrowed to within the few hours of development, the period of starvation recognition. The mutant is also defective in the production of A-factor, an early extracellular cell-density signal. The relA gene from Escherichia coli, which encodes a ribosome-dependent (p)ppGpp synthetase, rescues this mutant. We also demonstrate that inactivation of the M. xanthus relA homolog blocks development and the accumulation of (p)ppGpp. Moreover, the wild-type allele of Myxococcus relA rescues DK527. These observations support a model in which accumulation of (p)ppGpp, in response to starvation, initiates the program of fruiting body development, including the production of A-factor.

References

Feb 25, 1978·Journal of Molecular Biology·J M CamposD R Zusman
Mar 1, 1992·Genes & Development·S LiL J Shimkets
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A KuspaD Kaiser
Sep 1, 1986·Developmental Biology·L KroosD Kaiser
Sep 8, 1988·Nature·A CoulsonY Kohara
Dec 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·G S STENT, S BRENNER
Nov 14, 2006·Journal of Bacteriology·Oleksii SliusarenkoGeorge Oster

❮ Previous
Next ❯

Citations

Jun 23, 2006·Applied Microbiology and Biotechnology·Toshiyuki Ueki, Sumiko Inouye
Apr 4, 2000·Current Opinion in Microbiology·B A Lazazzera
Apr 3, 2001·Current Opinion in Microbiology·D Chatterji, A K Ojha
Mar 6, 1999·Current Opinion in Microbiology·G H Kelemen, M J Buttner
Mar 6, 1999·Current Opinion in Microbiology·D Kaiser
Sep 6, 2000·Molecular Microbiology·E W Crawford, L J Shimkets
Nov 13, 2001·Molecular Microbiology·D J WhiteP L Hartzell
Mar 29, 2002·Molecular Microbiology·Derek H Wells, Sharon R Long
Apr 23, 2002·Molecular Microbiology·Brian K HammerMichele S Swanson
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Krista M GiglioAnthony G Garza
Dec 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Paul J A ErbelKevin H Gardner
Jul 19, 2013·Proceedings. Biological Sciences·Luke M Noble, Alex Andrianopoulos
May 22, 2010·Science·Yuen-Tsu N YuGregory J Velicer
Sep 24, 2004·Infection and Immunity·David L EricksonDouglas G Storey
May 22, 2007·Journal of Bacteriology·James E Berleman, John R Kirby
May 13, 2008·Journal of Bacteriology·Lee Kroos
Nov 10, 2009·Journal of Bacteriology·Krista M GiglioAnthony G Garza
Oct 17, 2006·Journal of Bacteriology·Laurie N DiDonatoDerek R Lovley
Oct 2, 2007·Journal of Bacteriology·Christopher J Rosario, Mitchell Singer
Nov 13, 2007·Journal of Bacteriology·Xingqi ShiLotte Søgaard-Andersen
Jan 25, 2011·Journal of Bacteriology·Jun-Seok LeeLee Kroos
Mar 3, 2009·Journal of Bacteriology·Diana García-MorenoMontserrat Elías-Arnanz
Nov 14, 2000·Journal of Bacteriology·A G GarzaM Singer
Nov 8, 2001·Journal of Bacteriology·H Sun, W Shi
Sep 2, 2003·Journal of Bacteriology·Anders A Rasmussen, Lotte Søgaard-Andersen
Jan 4, 2003·Journal of Bacteriology·Marc B Concepcion, David R Nelson
May 20, 2004·Journal of Bacteriology·Margaret BrennerMitchell Singer
Jun 19, 2004·Journal of Bacteriology·Jimmy S JakobsenDale Kaiser

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.