The guanylyltransferase domain of mammalian mRNA capping enzyme binds to the phosphorylated carboxyl-terminal domain of RNA polymerase II.

The Journal of Biological Chemistry
C K HoS Shuman

Abstract

We have conducted a biochemical and genetic analysis of mouse mRNA capping enzyme (Mce1), a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities. The principal conclusions are as follows: (i) the mammalian capping enzyme consists of autonomous and nonoverlapping functional domains; (ii) the guanylyltransferase domain Mce1(211-597) is catalytically active in vitro and functional in vivo in yeast in lieu of the endogenous guanylyltransferase Ceg1; (iii) the guanylyltransferase domain per se binds to the phosphorylated RNA polymerase II carboxyl-terminal domain (CTD), whereas the triphosphatase domain, Mce1(1-210), does not bind to the CTD; and (iv) a mutation of the active site cysteine of the mouse triphosphatase elicits a strong growth-suppressive phenotype in yeast, conceivably by sequestering pre-mRNA ends in a nonproductive complex or by blocking access of the endogenous yeast triphosphatase to RNA polymerase II. These findings contribute to an emerging model of mRNA biogenesis wherein RNA processing enzymes are targeted to nascent polymerase II transcripts through contacts with the CTD. The phosphorylation-dependent interaction between guanylyltransferase and the CTD is conserve...Continue Reading

References

Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Shuman, J Hurwitz
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·S Shuman
Jul 1, 1995·Molecular and Cellular Biology·S Gunnery, M B Mathews
Mar 9, 1995·Nature·S M LiaoR A Young
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·L D Fresco, S Buratowski
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·B Schwer, S Shuman
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·A YuryevJ L Corden
Aug 9, 1996·The Journal of Biological Chemistry·M E Dahmus
Nov 1, 1996·Cell·J M DenuJ E Dixon
Nov 1, 1996·Genes & Development·G OrphanidesD Reinberg
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·S P WangS Shuman
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·Z YueA J Shatkin
Dec 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·S Shuman

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Citations

Dec 16, 2003·Journal of Molecular Biology·Benoît PalancadeMarie-Françoise Dubois
May 30, 2001·Molecular and Biochemical Parasitology·T Takagi, S Buratowski
Jan 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·D BarillàN J Proudfoot
Aug 20, 2010·The Journal of Biological Chemistry·Man-Hee SuhJianhua Fu
Jul 14, 2005·The Journal of Biological Chemistry·Daniel P MorrisDebra A Schwinn
Apr 29, 1999·Nucleic Acids Research·C R RodriguezS Buratowski
Jan 5, 2001·Nucleic Acids Research·Y PeiS Shuman
Oct 10, 2006·Nucleic Acids Research·Megan P Hall, C Kiong Ho
Jul 1, 1999·Genes & Development·H ChoD Reinberg
Jul 27, 1999·Genes & Development·Y Wen, A J Shatkin
May 3, 2006·Genes & Development·Rita DasRobin Reed
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·S McCrackenD Bentley
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S Shuman
Nov 29, 2002·Eukaryotic Cell·Toshimitsu TakagiStephen Buratowski
Feb 19, 2009·Molecular and Cellular Biology·Yuichi OtsukaDaniel R Schoenberg
Oct 5, 2001·Molecular and Cellular Biology·D P TranH G Martinson
Jul 1, 2004·Molecular and Cellular Biology·Hye-Jin KimEun-Jung Cho
Dec 5, 2012·PloS One·Jennifer A MitchellPeter Fraser
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Meisheng ZhouAjit Kumar
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y WenA J Shatkin
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·John W Stiller, Benjamin D Hall
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·T TakagiS Buratowski
May 21, 2009·The Plant Journal : for Cell and Molecular Biology·Daniel KierzkowskiArtur Jarmolowski

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