The gut microbiome restores intrinsic and extrinsic nerve function in germ-free mice accompanied by changes in calbindin

Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society
K A McVey NeufeldW Kunze

Abstract

The microbiome is essential for normal myenteric intrinsic primary afferent neuron (IPAN) excitability. These neurons control gut motility and modulate gut-brain signaling by exciting extrinsic afferent fibers innervating the enteric nervous system via an IPAN to extrinsic fiber sensory synapse. We investigated effects of germ-free (GF) status and conventionalization on extrinsic sensory fiber discharge in the mesenteric nerve bundle and IPAN electrophysiology, and compared these findings with those from specific pathogen-free (SPF) mice. As we have previously shown that the IPAN calcium-dependent slow afterhyperpolarization (sAHP) is enhanced in GF mice, we also examined the expression of the calcium-binding protein calbindin in these neurons in these different animal groups. IPAN sAHP and mesenteric nerve multiunit discharge were recorded using ex vivo jejunal gut segments from SPF, GF, or conventionalized (CONV) mice. IPANs were excited by adding 5 μM TRAM-34 to the serosal superfusate. We probed for calbindin expression using immunohistochemical techniques. SPF mice had a 21% increase in mesenteric nerve multiunit firing rate and CONV mice a 41% increase when IPANs were excited by TRAM-34. For GF mice, this increase was bar...Continue Reading

References

Aug 1, 1982·The Journal of Physiology·K MoritaT Tokimasa
Dec 2, 1999·Gastroenterology·C GiaroniG Frigo
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·H WulffK G Chandy
Feb 15, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·E HusebyeT Midtvedt
Jan 16, 2002·The Journal of Physiology·François RugieroNadine Clerc
Aug 31, 2002·Journal of Gastroenterology·Georg B T von BoyenJoachim Kirsch
Dec 4, 2003·Current Opinion in Pharmacology·Michael D Gershon
Feb 6, 2004·Journal of Neuroscience Methods·Robert J PhillipsTerry L Powley
Feb 7, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K KammM Schemann
Jun 3, 2004·Nature Reviews. Immunology·Andrew J Macpherson, Nicola L Harris
Jun 29, 2004·Trends in Genetics : TIG·Lakshminarayan M IyerEugene V Koonin
Aug 11, 2006·Journal of Neurophysiology·Yukang MaoWolfgang Kunze
May 19, 2007·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D FerensJ B Furness
Oct 16, 2008·Cell and Tissue Research·Zheng-Dong QuJohn B Furness
Feb 13, 2009·Journal of Cellular and Molecular Medicine·Wolfgang A KunzeJohn Bienenstock
May 20, 2009·The International Journal of Biochemistry & Cell Biology·Aaron James Camp, Rajiv Wijesinghe
Jun 4, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bingxian WangWolfgang A Kunze
Nov 9, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K M NeufeldJ A Foster
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rochellys Diaz HeijtzSven Pettersson
Jun 17, 2011·Nature·Andrew L KauJeffrey I Gordon
Aug 24, 2011·Annals of Nutrition & Metabolism·Fredrik Bäckhed
Aug 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Javier A BravoJohn F Cryan
Dec 14, 2011·Frontiers in Physiology·Sue GrenhamTimothy G Dinan
Sep 13, 2012·Nature Reviews. Neuroscience·John F Cryan, Timothy G Dinan
Sep 25, 2012·Nature Reviews. Microbiology·Stephen M CollinsPremysl Bercik
Sep 27, 2012·Current Opinion in Gastroenterology·Paul ForsytheJohn Bienenstock
Nov 10, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Azucena Perez-BurgosWolfgang Kunze
Nov 28, 2012·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·K A McVey NeufeldW A Kunze
Feb 7, 2013·Trends in Neurosciences·Jane A Foster, Karen-Anne McVey Neufeld
Dec 18, 2013·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J CollinsE M Ratcliffe
Apr 11, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Azucena Perez-BurgosWolfgang A Kunze
Sep 10, 2014·The Proceedings of the Nutrition Society·Elaine PattersonCatherine Stanton

❮ Previous
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Citations

Jan 19, 2016·Frontiers in Cellular Neuroscience·Tor C Savidge
Oct 2, 2015·Current Opinion in Pharmacology·Peter HolzerAitak Farzi
Feb 26, 2016·The International Journal of Neuropsychopharmacology·Pauline LuczynskiJohn F Cryan
Mar 22, 2016·Developmental Biology·Robert O Heuckeroth, Karl-Herbert Schäfer
May 29, 2016·Developmental Biology·M M HaoW Boesmans
Oct 22, 2016·Neuroscience and Biobehavioral Reviews·Karen-Anne McVey NeufeldJohn F Cryan
Jun 28, 2016·Developmental Biology·Niall P Hyland, John F Cryan
Jul 30, 2016·Mediators of Inflammation·Claudio BernardazziHeitor Siffert P de Souza
Dec 23, 2016·Cellular and Molecular Neurobiology·Petr M MasliukovJean-Pierre Timmermans
Jun 3, 2016·Canadian Journal of Psychiatry. Revue Canadienne De Psychiatrie·Sophie LeclercqJohn Bienenstock
Jan 11, 2018·International Journal of Molecular Sciences·Katharina ZetzmannAxel Brehmer
Dec 8, 2017·Frontiers in Immunology·Peter HolzerFlorian Reichmann
Sep 12, 2017·Environmental Microbiology·Aimée ParkerCarmen Pin
Aug 3, 2017·Annals of the New York Academy of Sciences·Eoin SherwinJohn F Cryan
May 16, 2018·Nature Reviews. Nephrology·Tao YangMohan K Raizada
Aug 3, 2018·Nature Reviews. Gastroenterology & Hepatology·Keith A SharkeyDerek M McKay
Jan 16, 2019·Current Protocols in Mouse Biology·Máximo E LangeG Douglas Inglis
Oct 16, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dervla O'Malley
Aug 29, 2019·Physiological Reviews·John F CryanTimothy G Dinan
Jun 6, 2018·Proceedings of the National Academy of Sciences of the United States of America·Filipe De VadderFredrik Bäckhed
Mar 7, 2019·Frontiers in Endocrinology·Mihai CovasaClaudiu Cobuz
Apr 3, 2019·International Journal of Molecular Sciences·Andreina BajCristina Giaroni
Sep 25, 2020·Nature·Helen E VuongElaine Y Hsiao
Mar 15, 2018·Frontiers in Neuroscience·Maria M Buckley, Dervla O'Malley
Apr 28, 2018·Nutrition Reviews·M Hasan MohajeriPeter Weber
Oct 28, 2019·Reviews in Endocrine & Metabolic Disorders·Estelle Grasset, Remy Burcelin
May 20, 2020·Cellular and Molecular Life Sciences : CMLS·Candice Fung, Pieter Vanden Berghe
Jul 27, 2018·Nature Reviews. Neuroscience·Meenakshi Rao, Michael D Gershon
Feb 6, 2017·Animal Health Research Reviews·Ian TizardSusan Payne
Jul 16, 2020·Soa--chʻŏngsonyŏn Chŏngsin Ŭihak = Journal of Child & Adolescent Psychiatry·Mira KimTae Won Park
Jul 16, 2020·Soa--chʻŏngsonyŏn Chŏngsin Ŭihak = Journal of Child & Adolescent Psychiatry·Donghun Oh, Keun-Ah Cheon
May 12, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Lin Y HungJaime P P Foong
Sep 10, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jaime P P FoongJoel C Bornstein
Jan 6, 2021·Cells·Verena Pawolski, Mirko H H Schmidt
Feb 11, 2021·International Journal of Molecular Sciences·Davide BanfiAndreina Baj
Dec 15, 2020·Trends in Microbiology·Amélie JolyFilipe De Vadder
Jun 15, 2021·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Candice FungPieter Vanden Berghe

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