PMID: 7544103Jul 1, 1995Paper

The gvpA/C cluster of Anabaena flos-aquae has multiple copies of a gene encoding GvpA

Archives of Microbiology
P K Hayes, R S Powell

Abstract

Southern analysis of genomic DNA from Anabaena flos-aquae revealed that the genes encoding the two authenticated protein components of cyanobacterial gas vesicles, GvpA and GvpC, were carried on the same 4.9-kb HindIII restriction fragment. By comparing the hybridization intensities observed when either gvpA- or gvpC-specific oligonucleotides are bound to this HindIII fragment, we calculated that the A. flos-aquae genome contains seven copies of gvpA and a single copy of gvpC. The nucleotide sequence of the longest cloned section of the gvpA/C cluster of A. flos-aquae DNA revealed the presence of four complete copies of gvpA and part of a fifth copy located upstream from a single copy of gvpC; no clones carrying the entire gvpA/C-bearing HindIII fragment were identified. The distribution of Sau3A restriction sites throughout the gvpA/C-bearing genomic HindIII fragment resembled that seen in the cloned portion of the gvpA/C cluster and is consistent with that expected for a cluster containing seven copies of gvpA and one copy of gvpC. The length of transcripts that hybridize to both gvpA and gvpC on Northern blots was consistent with a 7gvpA + 1gvpC transcriptional unit.

References

Jan 1, 1992·Archives of Microbiology·P K HayesA E Walsby
Mar 1, 1989·Journal of Bacteriology·T DamervalN Tandeau de Marsac
Dec 1, 1989·The Biochemical Journal·A E Walsby, P K Hayes
Nov 25, 1987·Nucleic Acids Research·G GrimaldiC Schneider
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T R CechP S Perlman
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen
Mar 1, 1994·Microbiological Reviews·A E Walsby

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Citations

Jun 29, 2016·ACS Nano·Anupama LakshmananMikhail G Shapiro
Sep 4, 2012·Nature Reviews. Microbiology·Felicitas Pfeifer
Mar 27, 2002·DNA Sequence : the Journal of DNA Sequencing and Mapping·D AlbouyN T De Marsac
Jan 9, 2016·Environmental Microbiology·Yosuke TashiroGeorge P C Salmond
Jun 5, 1998·Microbiology·Sonja OffnerFelicitas Pfeifer

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