The GYF domain protein PSIG1 dampens the induction of cell death during plant-pathogen interactions

PLoS Genetics
Hidenori MatsuiHirofumi Nakagami

Abstract

The induction of rapid cell death is an effective strategy for plants to restrict biotrophic and hemi-biotrophic pathogens at the infection site. However, activation of cell death comes at a high cost, as dead cells will no longer be available for defense responses nor general metabolic processes. In addition, necrotrophic pathogens that thrive on dead tissue, take advantage of cell death-triggering mechanisms. Mechanisms by which plants solve this conundrum remain described. Here, we identify PLANT SMY2-TYPE ILE-GYF DOMAIN-CONTAINING PROTEIN 1 (PSIG1) and show that PSIG1 helps to restrict cell death induction during pathogen infection. Inactivation of PSIG1 does not result in spontaneous lesions, and enhanced cell death in psig1 mutants is independent of salicylic acid (SA) biosynthesis or reactive oxygen species (ROS) production. Moreover, PSIG1 interacts with SMG7, which plays a role in nonsense-mediated RNA decay (NMD), and the smg7-4 mutant allele mimics the cell death phenotype of the psig1 mutants. Intriguingly, the psig1 mutants display enhanced susceptibility to the hemi-biotrophic bacterial pathogen. These findings point to the existence and importance of the SA- and ROS-independent cell death constraining mechanism a...Continue Reading

References

Mar 26, 1992·Nature·S H Lillie, S S Brown
May 1, 1990·The Plant Cell·E Koch, A Slusarenko
May 20, 1994·Cell·R A DietrichJ L Dangl
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·K NishizawaE L Reinherz
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Nov 7, 1999·Molecular Plant-microbe Interactions : MPMI·D J KliebensteinJ L Dangl
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·J DewdneyF M Ausubel
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·K S CenturyB J Staskawicz
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Miguel Angel TorresJonathan D G Jones
Feb 15, 2002·The Plant Journal : for Cell and Molecular Biology·Daniel H AvivJeffery L Dangl
Aug 2, 2002·Cell·Kyoung Hee Nam, Jianming Li
Apr 5, 2003·Current Biology : CB·Patrick ReichenbachJoachim Lingner
May 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Petra EppleJeffery L Dangl
Mar 6, 2004·Molecular Plant-microbe Interactions : MPMI·Richard O'ConnellBernard Dumas
Apr 24, 2004·The Journal of Biological Chemistry·Michael KoflerChristian Freund
Nov 18, 2004·Molecular Cell·Leonie Unterholzner, Elisa Izaurralde
Aug 27, 2005·Molecular & Cellular Proteomics : MCP·Michael KoflerChristian Freund
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Jun 23, 2007·Current Biology : CB·Birgit KemmerlingThorsten Nürnberger
Jul 13, 2007·Nature·Delphine ChinchillaThomas Boller
Oct 13, 2007·Autophagy·Shalaka Patel, Savithramma P Dinesh-Kumar
Apr 1, 2009·FEBS Letters·Naoyoshi KumakuraYuichiro Watanabe
May 21, 2009·The Plant Journal : for Cell and Molecular Biology·Maxwell S BushJohn H Doonan
Jun 2, 2009·Molecular & Cellular Proteomics : MCP·Michael KoflerChristian Freund
Jun 13, 2009·The Plant Journal : for Cell and Molecular Biology·Mari NarusakaYoshihiro Narusaka
Jun 10, 2010·Bioscience, Biotechnology, and Biochemistry·Shinya NakamuraSumie Ishiguro

❮ Previous
Next ❯

Citations

May 2, 2018·FEBS Letters·Tatsuya NoboriKenichi Tsuda
Mar 29, 2021·The FEBS Journal·Jeffrey R AllenLucia C Strader
Nov 11, 2021·The FEBS Journal·Mary Christie, Cátia Igreja

❮ Previous
Next ❯

Methods Mentioned

BETA
chemical treatments
Y2H
PCR
reverse transcription PCR
fluorescence microscopy

Software Mentioned

house script
MEGA6
Image
2DICAL
in
R
Photoshop
Mascot

Related Concepts

Related Feeds

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

© 2022 Meta ULC. All rights reserved