The GyrA encoded gene: A pertinent marker for the phylogenetic revision of Helicobacter genus

Systematic and Applied Microbiology
Armelle MénardFrancis Mégraud

Abstract

Phylogeny of Epsilonproteobacteria is based on sequencing of the 16S rRNA gene. However, this gene is not sufficiently discriminatory in Helicobacter species and alternative markers would be useful. In this study, the 16S rRNA, gyrA, hsp60, gyrB, and ureA-ureB gene sequences, as well as GyrA, HSP60 and GyrB protein sequences were analyzed as tools to support Helicobacter species phylogeny: 72 Helicobacter strains, belonging to 41 species of which 36 are validated species, were included. Results of the phylogenetic reconstructions of the GyrA gene encoded protein (approximately 730 residues) indicated the most stable trees to bootstrap resampling with a good separation of Helicobacter taxa, especially between gastric and enterohepatic species. Moreover, the GyrA tree revealed high similarity with that of the gyrB and ureA-ureB genes (restricted to urease-positive Helicobacter species). However, some differences in clustering were observed when compared to the hsp60 and 23S rRNA gene trees. Altogether, these revised phylogenies (except the 16S rRNA gene for enterohepatic Helicobacters) enabled reliable clustering of Helicobacter cinaedi and 'Flexispira' strains, determined a reliable position for Helicobacter mustelae (except the...Continue Reading

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Citations

Jul 7, 2016·Systematic and Applied Microbiology·Yoshiaki KawamuraTakaaki Akaike
Aug 18, 2016·Helicobacter·Chloë De WitteFreddy Haesebrouck
Jan 13, 2018·Frontiers in Microbiology·Nathalie L van der Mee-MarquetPatrice François
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Nov 13, 2018·Journal of Bone and Joint Infection·Joanna KedraJean-Marc Ziza
Jun 22, 2021·Critical Reviews in Microbiology·Sofia Ochoa, Luis Collado

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