PMID: 8736May 1, 1976

The haemolytic effect of phallolysin

Naunyn-Schmiedeberg's Archives of Pharmacology
R SeegerL Feulner

Abstract

Phallolysin from the toadstool, Amanita phalloides, is a basic protein that causes direct haemolysis of red cells. The dose-response curve is steep; the pH optimum is in the weakly acid range. The rate of haemolysis increases with the concentration of the lysin, the optimal temperature is 20 degrees C. The percentage haemolysis-time curves are S-shaped. Haemolysis is of the non-osmotic type. Ca2+ is not required but inhibits haemolysis in a concentration-dependent fashion, as do Mg2+ and Zn2+. The red cell sensitivity of various animal species decreases in the following sequence:mouse greater than rabbit = guniea pig greater than rat greater than man greater than dog approximately or equal to pig greater than sheep = cattle. Red cells of cattle and sheep are largely resistant. Phallolysin is virtually not consumed on haemalysis: the amount of haemoglobin released increases with the number of red cells applied; on repeated addition of fresh red cells the haemolysate retains its full activity. Phallolysin is not inhibited by serum, albumin, cholesterol, lecithin, cephalin or sphingomyelin; inhibition by red cell ghosts of phallolysin haemolysis is considerably less than that of digitonin haemolysis. At sublytic concentrations pha...Continue Reading

References

Apr 3, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·R Seeger
Jan 1, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·R Seeger
Sep 24, 1975·Naunyn-Schmiedeberg's Archives of Pharmacology·K P OdenthalG Vogel
Feb 2, 1971·Biochimica Et Biophysica Acta·K S Cho, P Proulx
Jun 1, 1969·Toxicon : Official Journal of the International Society on Toxinology·T E Keen, H D Crone
Nov 1, 1969·Journal of Pharmaceutical Sciences·T Kondo, M Tomizawa
Aug 1, 1969·Journal of Medical Microbiology·M R Wright
Apr 1, 1968·Chemical & Pharmaceutical Bulletin·T Kondo, M Tomizawa
Feb 1, 1966·Journal of Colloid and Interface Science·T Kondo, M Tomizawa
Mar 26, 1956·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·E RIDEAL, F H TAYLOR
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Citations

Jan 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·R Seeger, E Bunsen
Feb 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·R Seeger
Mar 31, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·M Frimmer
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·M P Merker, L Levine
Jan 1, 1987·Toxicon : Official Journal of the International Society on Toxinology·A W Bernheimer, J D Oppenheim
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W T Shier
Jul 10, 2012·Medical Mycology·Ajay P NayakDonald H Beezhold
Sep 27, 1976·Zeitschrift Für Lebensmittel-Untersuchung Und -Forschung·R SeegerS Schönhut

Related Concepts

Amanitins
Metazoa
Cations, Divalent
Bos indicus
Dose-Response Relationship, Drug
Erythrocytes
Cavia porcellus
Eryhem
Hemolysin
Hemolysis

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