The Hampering Effect of Heavy Water (D2 O) on Oscillatory Peptidization of Selected Proteinogenic α-Amino Acids

Frontiers in Chemistry
Agnieszka FulczykMieczysław Sajewicz

Abstract

We present an overview of our studies on the hampering effect of heavy water (D2O) on spontaneous oscillatory peptidization of selected proteinogenic α-amino acids. The investigated set of compounds included three endogenous and two exogenous species. The experiments were carried out with use of high-performance liquid chromatography (HPLC), mass spectrometry (MS) and scanning electron microscopy (SEM). These techniques were chosen to demonstrate spontaneous oscillatory peptidization of α-amino acids in an absence of D2O (HPLC) and the hampering effect of D2O on peptidization (HPLC, MS and SEM). The HPLC analyses were carried out at 21 ± 0.5°C with each α-amino acid freshly dissolved in the binary liquid mixture of organic solvent + H2O, 70:30 (v/v) or in pure D2O for several dozen hours or several hours, respectively. The analyses with use of MS and SEM were carried out, respectively, after 7 days and 1 month of sample storage period in the darkness at 21 ± 0.5°C and for these experiments, each α-amino acid was dissolved in the liquid mixture of organic solvent + X, 70:30 (v/v), where X: H2O + D2O in volume proportions from 30:0 to 0:30. The results obtained with use of HPLC, MS and SEM point out to the strong hampering effect...Continue Reading

References

Nov 1, 1962·The American Journal of Physiology·J J KATZA J FINKEL
Feb 16, 1934·Science·G N Lewis
Sep 1, 2009·The Journal of Physical Chemistry. a·Angela G RincónA J Colussi
Oct 16, 2012·Physical Chemistry Chemical Physics : PCCP·Michael StichDavid Hochberg
Apr 11, 2013·Journal of Chromatographic Science·Mieczysław SajewiczTeresa Kowalska
Nov 3, 2015·Current Protein & Peptide Science·Agnieszka GodziekTeresa Kowalska
May 18, 2016·Journal of Chromatographic Science·Anna MaciejowskaIrving R Epstein

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Methods Mentioned

BETA
scanning electron microscopy

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