The Hanahan Method for Preparation and Transformation of Competent Escherichia coli : High-Efficiency Transformation

Cold Spring Harbor Protocols
Michael R Green, Joseph Sambrook

Abstract

Chemical transformation is a highly efficient, commonly used method to transform Escherichia coli with plasmid DNA. Hanahan's procedure works well with many strains of E. coli commonly used in molecular cloning. Many variations on the basic technique have been described, all directed toward optimizing the efficiency of transformation. It is now possible on a routine basis to achieve transformation frequencies ranging from 106 to 109 transformants/µg of superhelical plasmid DNA.

References

Jan 1, 1991·Methods in Enzymology·D HanahanF R Bloom
Jun 5, 1983·Journal of Molecular Biology·D Hanahan

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Citations

Jun 3, 2020·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Feb 4, 2021·Microbial Biotechnology·João Heitor Colombelli Manfrão-NettoMagnus Carlquist
Nov 4, 2020·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Nov 5, 2020·Tissue Engineering and Regenerative Medicine·Tamilmahan ParamasivamNaveen Kumar
Feb 7, 2021·Microorganisms·João Heitor Colombelli Manfrão-NettoNádia Skorupa Parachin
Nov 4, 2020·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Apr 3, 2021·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Apr 3, 2021·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Jun 3, 2021·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook
Nov 3, 2021·Cold Spring Harbor Protocols·Michael R Green, Joseph Sambrook

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