The HARE chip for efficient time-resolved serial synchrotron crystallography.

Journal of Synchrotron Radiation
Pedram MehrabiR J Dwayne Miller

Abstract

Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.

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Citations

Sep 18, 2020·IUCrJ·Patrick RabeRobin L Owen
Jan 9, 2021·Quarterly Reviews of Biophysics·Marcus Fischer
Aug 28, 2020·Current Opinion in Structural Biology·Arwen R Pearson, Pedram Mehrabi
Feb 10, 2021·Acta Crystallographica. Section D, Structural Biology·Claudia StohrerArwen R Pearson
May 6, 2021·Acta Crystallographica. Section D, Structural Biology·Gabrielle IllavaRobert E Thorne
Jun 3, 2021·Acta Crystallographica. Section D, Structural Biology·Brenna Norton-BakerEike C Schulz
Aug 28, 2021·Science·Gisela Brändén, Richard Neutze
Sep 3, 2021·Acta Crystallographica. Section D, Structural Biology·Isabelle MartielCelestino Padeste

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Methods Mentioned

BETA
X-ray
chip
chips
PCR

Software Mentioned

LAMA
HARE

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