The haustorial transcriptomes of Uromyces appendiculatus and Phakopsora pachyrhizi and their candidate effector families

Molecular Plant Pathology
Tobias LinkSteven A Whitham

Abstract

Haustoria of biotrophic rust fungi are responsible for the uptake of nutrients from their hosts and for the production of secreted proteins, known as effectors, which modulate the host immune system. The identification of the transcriptome of haustoria and an understanding of the functions of expressed genes therefore hold essential keys for the elucidation of fungus-plant interactions and the development of novel fungal control strategies. Here, we purified haustoria from infected leaves and used 454 sequencing to examine the haustorial transcriptomes of Phakopsora pachyrhizi and Uromyces appendiculatus, the causal agents of soybean rust and common bean rust, respectively. These pathogens cause extensive yield losses in their respective legume crop hosts. A series of analyses were used to annotate expressed sequences, including transposable elements and viruses, to predict secreted proteins from the assembled sequences and to identify families of candidate effectors. This work provides a foundation for the comparative analysis of haustorial gene expression with further insights into physiology and effector evolution.

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Citations

May 23, 2014·Annual Review of Phytopathology·Jessie FernandezRichard A Wilson
Sep 26, 2015·International Journal of Molecular Sciences·Daniela HirschburgerTobias Link
Dec 24, 2015·Frontiers in Plant Science·Cécile LorrainSébastien Duplessis
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Jul 1, 2016·Annual Review of Phytopathology·Steven A WhithamTarek Hewezi
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Apr 25, 2017·Phytopathology·Bret Cooper, Kimberly B Campbell
Nov 17, 2017·Molecular Plant-microbe Interactions : MPMI·Mingsheng QiSteven A Whitham
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Sep 15, 2020·World Journal of Microbiology & Biotechnology·Rajdeep JaswalT R Sharma
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