The hedgehog and Wnt/β-catenin system machinery mediate myofibroblast differentiation of LR-MSCs in pulmonary fibrogenesis

Cell Death & Disease
Xiang ChenXiaodong Han

Abstract

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive and fatal lung disease that is characterized by enhanced changes in stem cell differentiation and fibroblast proliferation. Resident mesenchymal stem cells (LR-MSCs) can undergo phenotype conversion to myofibroblasts to augment extracellular matrix production, impairing function and contributing to pulmonary fibrosis. Hedgehog and Wnt signaling are developmental signal cascades that play an essential role in regulating embryogenesis and tissue homeostasis. Recently, it has been reported that both hedgehog and Wnt signaling play important roles in pulmonary fibrogenesis. Thus, the identification of specific target regulators may yield new strategy for pulmonary fibrosis therapies. In our work, we demonstrated the critical role of Gli1, Wnt7b, Wnt10a and Fzd10 in the process of pulmonary fibrogenesis in vitro and in vivo. Gli1 was induced in LR-MSCs following TGF-β1 treatment and fibrotic lung tissues. Inhibition of Gli1 suppressed myofibroblast differentiation of LR-MSCs and pulmonary fibrosis, and decreased the expression of Wnt7b, Wnt10a and β-catenin. Gli1 bound to and increased promoter activity of the Wnt7b and Wnt10a genes, and Wnt7b and Wnt10a were critical act...Continue Reading

References

Feb 15, 2001·Annals of Internal Medicine·M SelmanUNKNOWN American College of Chest Physicians
Aug 25, 2001·The New England Journal of Medicine·T J Gross, G W Hunninghake
Oct 6, 2001·American Journal of Respiratory and Critical Care Medicine·T E KingR M Cherniack
Jan 25, 2002·Respiratory Research·Jack GauldiePatricia J Sime
Jul 18, 2002·American Journal of Respiratory and Critical Care Medicine·Andrew G NicholsonAthol U Wells
Oct 31, 2002·Development·Jianwu Bai, Denise Montell
Apr 23, 2003·The American Journal of Pathology·Edward E Morrisey
Apr 23, 2003·The American Journal of Pathology·Marco ChilosiClaudio Doglioni
Jun 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Luis A OrtizDonald G Phinney
Jan 30, 2004·Annual Review of Medicine·Victor J ThannickalFernando J Martinez
Sep 17, 2004·Nature Reviews. Genetics·Randall T MoonAjamete Kaykas
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Catriona Y Logan, Roel Nusse
Jan 5, 2005·Nature Reviews. Immunology·Frank J T Staal, Hans C Clevers
Feb 3, 2005·Cell Research·Roel Nusse
Sep 17, 2005·American Journal of Respiratory and Critical Care Medicine·Moises SelmanAlbert Zlotnik
May 23, 2006·Current Molecular Medicine·Freddy RadtkeOrbicia Riccio
May 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Matthias LauthRune Toftgård
May 15, 2007·American Journal of Respiratory and Critical Care Medicine·Jeffrey L SpeesArnold R Brody
Dec 18, 2007·Cell·Bryan T MacdonaldXi He
Mar 6, 2008·PLoS Medicine·Moisés SelmanNaftali Kaminski
Mar 15, 2008·Current Opinion in Cell Biology·He Huang, Xi He
May 15, 2008·PloS One·Melanie KönigshoffOliver Eickelberg
Mar 20, 2009·Journal of the American Society of Nephrology : JASN·Weichun HeYouhua Liu
Sep 8, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Keith D SalazarArnold R Brody
Sep 9, 2009·Development·Renée van Amerongen, Roel Nusse
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jynho KimPhilip A Beachy
Jun 10, 2010·Development·Christopher W Wilson, Pao-Tien Chuang
Jul 31, 2012·Respiratory Research·Travis MeutenPhilip L Sannes
Oct 2, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alfredo Lozano BolañosMoisés Selman
Oct 18, 2012·Science Signaling·David J RobbinsNatalia A Riobo
Mar 15, 2013·Bulletin of Experimental Biology and Medicine·E G SkurikhinA M Dygai
Jan 24, 2014·American Journal of Respiratory Cell and Molecular Biology·Elika Farrokhi MoshaiArnaud A Mailleux
Apr 14, 2016·Respiratory Research·Keishi OdaHiroshi Mukae
Nov 11, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Fujun YuJianjian Zheng
Feb 6, 2017·Experimental Cell Research·Xiqian LanPravin C Singhal

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Citations

Jul 13, 2019·Inflammation·Paolo CameliElena Bargagli
May 29, 2020·International Journal of Immunopathology and Pharmacology·Min-Na DongMing-Wei Liu
May 14, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stefanie Noel Sveiven, Tara M Nordgren
Jun 14, 2019·Stem Cells International·C Randall HarrellVladislav Volarevic
Nov 25, 2020·Molecular Biology Reports·Yue FangPengxiu Cao
May 18, 2021·Frontiers in Immunology·Chiel van GeffenSaeed Kolahian

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Methods Mentioned

BETA
flow cytometry
PCR
immunoprecipitation
pull-down
nuclear translocation
transfection
electrophoresis
ChIP
Co-IP

Software Mentioned

SPASS

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