The Helicase PIF1 Facilitates Resection over Sequences Prone to Forming G4 Structures

Cell Reports
Sonia JimenoPablo Huertas

Abstract

DNA breaks are complex lesions that can be repaired either by non-homologous end joining (NHEJ) or by homologous recombination (HR). The decision between these two routes of DNA repair is a key point of the DNA damage response (DDR) that is controlled by DNA resection. The core machinery catalyzing the resection process is well established. However, little is known about the additional requirements of DNA resection over DNA structures with high complexity. Here, we found evidence that the human helicase PIF1 has a role in DNA resection, specifically for defined DNA regions, such as those prone to form G-quadruplexes. Indeed, PIF1 is recruited to the site of DNA damage and physically interacts with proteins involved in DNA resection, and its depletion causes DNA damage sensitivity and a reduction of HR efficiency. Moreover, G4 stabilization by itself hampers DNA resection, a phenomenon suppressed by PIF1 overexpression.

Citations

Nov 28, 2019·Nature Communications·Nannan SuHaiwei Song
Oct 13, 2020·Nucleic Acids Research·Ke-Wei ZhengZheng Tan
Jan 29, 2021·Biochimica Et Biophysica Acta. General Subjects·Nitu Kumari, Sathees C Raghavan
Feb 18, 2021·Cell Chemical Biology·Pauline LejaultDavid Monchaud
Dec 11, 2020·RNA·Rosario Prados-CarvajalPablo Huertas
Mar 17, 2020·Current Opinion in Genetics & Development·Susanna Stroik, Eric A Hendrickson
Feb 12, 2019·DNA Repair·Wezley C Griffin, Michael A Trakselis
Jul 11, 2020·DNA Repair·Susanna Stroik, Eric A Hendrickson
May 20, 2021·Nucleic Acids Research·Phong Lan Thao TranVincent Croquette
Sep 1, 2021·Current Opinion in Genetics & Development·Erica J Polleys, Catherine H Freudenreich

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