The Helicobacter pylori autotransporter ImaA (HP0289) modulates the immune response and contributes to host colonization.

Infection and Immunity
William E SauseKaren M Ottemann

Abstract

The human pathogen Helicobacter pylori employs a diverse collection of outer membrane proteins to colonize, persist, and drive disease within the acidic gastric environment. In this study, we sought to elucidate the function of the host-induced gene HP0289, which encodes an uncharacterized outer membrane protein. We first generated an isogenic H. pylori mutant that lacks HP0289 and found that the mutant has a colonization defect in single-strain infections and is greatly outcompeted in mouse coinfection experiments with wild-type H. pylori. Furthermore, we used protease assays and biochemical fractionation coupled with an HP0289-targeted peptide antibody to verify that the HP0289 protein resides in the outer membrane. Our previous findings showed that the HP0289 promoter is upregulated in the mouse stomach, and here we demonstrate that HP0289 expression is induced under acidic conditions in an ArsRS-dependent manner. Finally, we have shown that the HP0289 mutant induces greater expression of the chemokine interleukin-8 (IL-8) and the cytokine tumor necrosis factor alpha (TNF-α) in gastric carcinoma cells (AGS). Similarly, transcription of the IL-8 homolog keratinocyte-derived chemokine (KC) is elevated in murine infections with...Continue Reading

References

Jun 17, 1995·Lancet·E J KuipersS G Meuwissen
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A CovacciN Figura
Nov 1, 1995·Molecular Microbiology·A Camilli, J J Mekalanos
Aug 15, 1997·The Journal of Clinical Investigation·M J Blaser
Apr 16, 1998·Molecular Microbiology·A M LoweR L Deresiewicz
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·J SchultzC P Ponting
Mar 29, 2000·Journal of Bacteriology·D Beier, R Frank
Apr 14, 2000·The Journal of Infectious Diseases·A S DayP M Sherman
Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y YamaokaD Y Graham
Sep 6, 2000·Molecular Microbiology·J P DonahueG G Miller
Feb 17, 2001·Infection and Immunity·I R Henderson, J P Nataro
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L F LinH Kong
Jun 5, 2001·Nature Reviews. Molecular Cell Biology·C Montecucco, R Rappuoli
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·S H LeeA Camilli
Dec 26, 2001·Nucleic Acids Research·Alex BatemanErik L L Sonnhammer
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 16, 2002·Infection and Immunity·Karen M Ottemann, Andrew C Lowenthal
Apr 25, 2002·Nucleic Acids Research·Irina NazarenkoAyoub Rashtchian
Oct 11, 2002·The New England Journal of Medicine·Sebastian Suerbaum, Pierre Michetti
Apr 2, 2003·The Journal of Experimental Medicine·Holger KavermannRainer Haas
May 23, 2003·Infection and Immunity·D Scott MerrellStanley Falkow
Dec 17, 2003·The Journal of Experimental Medicine·Marianna BoncristianoCosima T Baldari
Apr 28, 2004·FEMS Microbiology Letters·Michael PflockDagmar Beier
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak

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Citations

Jun 9, 2016·Toxins·Nora J FoegedingTimothy L Cover
Feb 7, 2015·PLoS Pathogens·Nina Bertaux-SkeirikYana Zavros
Nov 1, 2016·Infection and Immunity·William E SauseKaren M Ottemann
Apr 29, 2014·Journal of Bacteriology·Bradley J VossTimothy L Cover

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