The helper-component protease transmission factor of tobacco etch potyvirus binds specifically to an aphid ribosomal protein homologous to the laminin receptor precursor

The Journal of General Virology
Lourdes Fernández-CalvinoJ J Lopez-Moya

Abstract

Potyviruses are plant pathogens transmitted by aphids in a non-persistent manner. During transmission, the virus-encoded factor helper-component protease (HCPro) is presumed to act as a molecular bridge, mediating the reversible retention of virions to uncharacterized binding sites in the vector mouthparts. Whilst the predicted interaction between HCPro and the coat protein (CP) of virions has been confirmed experimentally, the characterization of putative HCPro-specific receptors in aphids has remained elusive, with the exception of a report that described binding of HCPro of zucchini yellow mosaic virus to several cuticle proteins. To identify other aphid components that could play a role during transmission, this study used purified HCPro of tobacco etch virus (TEV) in far-Western blotting assays as bait to select interactors among proteins extracted from aphid heads. With this approach, new HCPro-interacting proteins were found, and several were identified after mass spectrometry analysis and searches in databases dedicated to aphid sequences. Among these interactors, a ribosomal protein S2 (RPS2) was chosen for further investigation due to its homology with the laminin receptor precursor, known to act as the receptor of se...Continue Reading

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Citations

Jul 27, 2011·Current Opinion in Microbiology·Stéphane BlancMartin Drucker
May 18, 2018·Journal of Virology·Craig G WebsterMarilyne Uzest
Jan 26, 2020·Viruses·Adrian J GibbsRoger A C Jones
Jul 28, 2020·Viruses·Kiran R GadhaveRajagopalbabu Srinivasan

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