The helping hand of collagenase-3 (MMP-13): 2.7 A crystal structure of its C-terminal haemopexin-like domain

Journal of Molecular Biology
F X Gomis-RüthW Bode

Abstract

Collagenase-3 (MMP-13) is a matrix metalloproteinase involved in human breast cancer pathology and in arthritic processes. The crystal structure of its C-terminal haemopexin-like domain has been solved by molecular replacement and refined to an R-value of 0.195 using data to 2.7 A resolution. This structure reveals a disk-like shape. The chain is folded into a beta-propeller structure of pseudo 4-fold symmetry, with the four propeller blades arranged around a funnel-like tunnel. This central tunnel tube harbours four ions assigned as two calcium and two chloride ions. The C-terminal domain of collagenase-3 has a similar structure to the equivalent domain of gelatinase A and fibroblast collagenase 1; however, its detailed structure and surface charge pattern has a somewhat greater similarity to the latter, in agreement with the subgrouping of MMP-13 with the collagenase subfamily of MMPs. It is proposed that several small structural differences may act together to confer the characteristic binding and cleavage specificities of collagenases for triple-helical substrates, probably in co-operation with a fitting interdomain linker.

Citations

Feb 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shiamalee PerumalJoseph P R O Orgel
Jun 4, 2002·DNA and Cell Biology·Emanuela Tolosano, Fiorella Altruda
Feb 12, 2010·Plant Physiology·Vineet GaurDinakar M Salunke
Feb 9, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Vineet GaurDinakar M Salunke
Jul 23, 1999·Annals of the New York Academy of Sciences·W BodeK Maskos
Apr 25, 2009·Journal of Biomechanical Engineering·Karla E-K WyattPeter A Torzilli
Apr 8, 2006·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Jirí KukackaVáclav Pelouch
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina MorgunovaKarl Tryggvason
Jul 17, 2004·The EMBO Journal·Linda ChungHideaki Nagase
Aug 24, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S P ScullyJ M Harrelson
Jun 14, 2005·Journal of Molecular Biology·Rune I LindstadJan-Olof Winberg
Oct 11, 2005·Protein Expression and Purification·Justyna MysliwyJoachim Grötzinger
Apr 13, 2018·Expert Review of Proteomics·Mustapha BenkhalifaMonia Allal-Elasmi
Jul 27, 2004·Protein Science : a Publication of the Protein Society·Swarnalatha Y Reddy, Thomas C Bruice
Nov 4, 2010·Journal of Neurochemistry·Eun-Mee Kim, Onyou Hwang
Sep 27, 2000·FEMS Microbiology Letters·S J CrennellR H ffrench-Constant
Aug 6, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Enrico A SturaHideaki Nagase
May 23, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tania R LizarbeCarlos Zaragoza
Mar 27, 2003·Periodontology 2000·Veli-Jukka UittoCristopher McCulloch
Dec 12, 2012·Journal of Computer-aided Molecular Design·Lingling GengBozhen Chen
Nov 23, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hong-Meng ChuangHorng-Jyh Harn
Oct 18, 2002·The Journal of Biological Chemistry·Elke RoebJoachim Grötzinger
Jul 27, 1999·The Journal of Biological Chemistry·H Nagase, J F Woessner
Nov 20, 1998·Breast Cancer Research and Treatment·C BenaudE W Thompson
Jul 8, 2005·The Journal of Biological Chemistry·David K RheeMatthew L Warman
Apr 28, 1999·Journal of Bacteriology·C M JungA Okabe
Dec 22, 2004·The Journal of Biological Chemistry·Daniela JozicKlaus Maskos
Oct 24, 1998·Progress in Biophysics and Molecular Biology·N Borkakoti

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