The Heme-Lys Cross-Link in Cytochrome P460 Promotes Catalysis by Enforcing Secondary Coordination Sphere Architecture.

Biochemistry
Rachael E ColemanKyle M Lancaster

Abstract

Cytochrome (cyt) P460 is a c-type monoheme enzyme found in ammonia-oxidizing bacteria (AOB) and methanotrophs; additionally, genes encoding it have been found in some pathogenic bacteria. Cyt P460 is defined by a unique post-translational modification to the heme macrocycle, where a lysine (Lys) residue covalently attaches to the 13' meso carbon of the porphyrin, modifying this heme macrocycle into the enzyme's eponymous P460 cofactor, similar to the cofactor found in the enzyme hydroxylamine oxidoreductase. This cross-link imbues the protein with unique spectroscopic properties, the most obvious of which is the enzyme's green color in solution. Cyt P460 from the AOB Nitrosomonas europaea is a homodimeric redox enzyme that produces nitrous oxide (N2O) from 2 equiv of hydroxylamine. Mutation of the Lys cross-link results in spectroscopic features that are more similar to those of standard cyt c' proteins and renders the enzyme catalytically incompetent for NH2OH oxidation. Recently, the necessity of a second-sphere glutamate (Glu) residue for redox catalysis was established; it plausibly serves as proton relay during the first oxidative half of the catalytic cycle. Herein, we report the first crystal structure of a cross-link de...Continue Reading

References

Mar 1, 1984·The Biochemical Journal·G Williamson, P C Engel
Mar 3, 1999·Biochemistry·J G MaJ A Shelnutt
Mar 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tatjana Kh ShokhirevaF Ann Walker
Oct 22, 2003·Journal of Magnetic Resonance·Adina P Golombek, Michael P Hendrich
Feb 13, 2007·FEBS Letters·Bradley O ElmoreAlan B Hooper
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Robert G KranzElaine R Frawley
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Jun 25, 2010·Journal of the American Chemical Society·Matthew D LiptakKara L Bren
Dec 5, 2013·The Journal of Biological Chemistry·Wouter J MaalckeBoran Kartal
Apr 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yuhan SunPaul M Champion
May 16, 2014·Journal of the American Chemical Society·Jonathan D CarantoDonald M Kurtz
Jul 12, 2014·Annual Review of Analytical Chemistry·Jean-Michel Savéant
Jun 27, 2015·Accounts of Chemical Research·Andrew P Hunt, Nicolai Lehnert
Apr 2, 2016·The Journal of Physical Chemistry. B·Amanda B GravesMatthew Denis Liptak
Nov 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jonathan D CarantoKyle M Lancaster
Jul 19, 2017·Proceedings of the National Academy of Sciences of the United States of America·Jonathan D Caranto, Kyle M Lancaster
Feb 6, 2018·Nature Reviews. Microbiology·Marcel M M KuypersBoran Kartal
Feb 29, 2020·Chemical Reviews·Christina FerousiKyle M Lancaster

❮ Previous
Next ❯

Citations

Nov 13, 2020·Accounts of Chemical Research·Rachael E Coleman, Kyle M Lancaster

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.