The hemicellulose-degrading enzyme system of the thermophilic bacterium Clostridium stercorarium : comparative characterisation and addition of new hemicellulolytic glycoside hydrolases

Biotechnology for Biofuels
J BroekerVladimir V Zverlov

Abstract

The bioconversion of lignocellulosic biomass in various industrial processes, such as the production of biofuels, requires the degradation of hemicellulose. Clostridium stercorarium is a thermophilic bacterium, well known for its outstanding hemicellulose-degrading capability. Its genome comprises about 50 genes for partially still uncharacterised thermostable hemicellulolytic enzymes. These are promising candidates for industrial applications. To reveal the hemicellulose-degrading potential of 50 glycoside hydrolases, they were recombinantly produced and characterised. 46 of them were identified in the secretome of C. stercorarium cultivated on cellobiose. Xylanases Xyn11A, Xyn10B, Xyn10C, and cellulase Cel9Z were among the most abundant proteins. The secretome of C. stercorarium was active on xylan, β-glucan, xyloglucan, galactan, and glucomannan. In addition, the recombinant enzymes hydrolysed arabinan, mannan, and galactomannan. 20 enzymes are newly described, degrading xylan, galactan, arabinan, mannan, and aryl-glycosides of β-d-xylose, β-d-glucose, β-d-galactose, α-l-arabinofuranose, α-l-rhamnose, β-d-glucuronic acid, and N-acetyl-β-d-glucosamine. The activities of three enzymes with non-classified glycoside hydrolase (G...Continue Reading

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Citations

Nov 6, 2018·Folia Microbiologica·Bhagwan Rekadwad, Juan M Gonzalez
Oct 20, 2019·Applied Microbiology and Biotechnology·Priyanka Bajaj, Ritu Mahajan
Nov 16, 2019·World Journal of Microbiology & Biotechnology·Samkelo MalgasBrett I Pletschke
Jul 1, 2020·Frontiers in Bioengineering and Biotechnology·Daochen ZhuJianzhong Sun
Dec 15, 2020·International Journal of Biological Macromolecules·Nisha SinghMunish Puri
Sep 20, 2020·Applied and Environmental Microbiology·Maria João Maurício da FonsecaYves Briers

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Methods Mentioned

BETA
Protein Assay
electrophoresis
acetylation
PCR
Thin-layer chromatography

Software Mentioned

ExPASy ProtParam
SignalP
MaxQuant

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