The hen's egg test for micronucleus induction (HET-MN): novel analyses with a series of well-characterized substances support the further evaluation of the test system

Mutation Research
Thorsten WolfNiels-Peter Luepke

Abstract

The hen's egg test for micronucleus induction (HET-MN) combines the use of the commonly accepted genetic endpoint "formation of micronuclei" with the well-characterized and complex model of the incubated hen's egg, which enables metabolic activation, elimination and excretion of xenobiotics -- including those that are mutagens or promutagens. This assay procedure is in line with demands for animal protection. In three previous publications we presented the scientific rationale and methodological aspects for this assay as well as results for some well-characterized mutagens and promutagens. Here we present the results of new experiments involving further genotoxic and non-genotoxic model substances. Making a comparison with published data we have to date not found any false negatives or false positives in the experiments presented here and in trials published before, thus demonstrating a promising predictivity of genotoxic effects with this assay. We could confirm relevant genotoxicity for the following substances in the HET-MN: acetylamino-fluorene (2-AAF), acrylamide (ACM), cytarabine (AraC), methotrexate (MTX), cadmium chloride (CD), dipotassium monochromate (DPC), and epirubicine (EPI). Negative results were obtained for azo...Continue Reading

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Citations

Sep 17, 2010·Nutrition Research Reviews·Pınar Erkekoğlu, Terken Baydar
Aug 11, 2012·Journal of Medicinal Food·Nancy ArgüellesEduardo Madrigal-Bujaidar
May 3, 2011·Archives of Toxicology·Sarah AdlerJosé-Manuel Zaldivar
Jun 29, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Gary M WilliamsUlrich Deschl
Mar 14, 2013·Critical Reviews in Toxicology·Larry D Kier, David J Kirkland
Oct 11, 2014·PloS One·Sabry Mohamed AttiaSaleh Abdulrahman Bakheet
Dec 7, 2011·Critical Reviews in Toxicology·Tatyana Y DoktorovaVera Rogiers
Aug 25, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Raffaella Corvi, Federica Madia
Jul 10, 2019·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·B Çelik, H Özparlak
Jun 4, 2021·Mutagenesis·Kerstin ReisingerRalph Pirow

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