The HepaRG cell line: a unique in vitro tool for understanding drug metabolism and toxicology in human

Expert Opinion on Drug Metabolism & Toxicology
Tommy B AnderssonJohn Gerry Kenna

Abstract

HepaRG is a unique cell line showing a great plasticity, which differentiates to both canaliculae-like and hepatocyte-like cells. The long-term stability of key cell functions, for example, the drug-metabolizing cytochrome P450 (CYP) enzyme activities, in culture is especially useful in drug metabolism, disposition and toxicity studies. This review describes features of the HepaRG cells focusing on drug-metabolizing enzymes and drug transporters, their functionality and regulation. Several applications in drug discovery studies are discussed and the use of HepaRG, as a human relevant predictive in vitro CYP induction model, is described. In addition, promising studies using HepaRG cells for understanding liver toxicity mechanisms by drug compounds are also discussed. HepaRG cells exhibit features which make them useful as an in vitro model for drug metabolism, disposition and toxicity studies, and could, for many studies, replace the requirement for primary human hepatocytes. Care should be taken since HepaRG cells are of a specific genotype which is reflected in the expression of drug processing proteins. The finding that HepaRG cells form tight junctions provides the basis for formation of functional canalicular structures an...Continue Reading

References

Nov 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Philippe GriponChristiane Guguen-Guillouzo
Aug 2, 2003·The New England Journal of Medicine·William M Lee
Apr 2, 2004·Gastroenterology·Romain ParentMarie-Anne Petit
Oct 6, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Caroline AninatAndré Guillouzo
Feb 21, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Marc Le VeeOlivier Fardel
May 23, 2006·FEBS Letters·Joseph AntounEmmanuelle Plée-Gautier
Mar 30, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Virginie CerecAnne Corlu
Oct 24, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Kajsa P Kanebratt, Tommy B Andersson
Nov 10, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Marc Le VeeOlivier Fardel
Jan 29, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Elise PetitFabrice Morel
Mar 19, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Rozenn JosséAndré Guillouzo
Apr 4, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Kajsa P Kanebratt, Tommy B Andersson
Mar 31, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Miia TurpeinenOlavi Pelkonen
Aug 22, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Claire LegendreFabrice Morel
Sep 30, 2009·Expert Opinion on Drug Metabolism & Toxicology·Olivier Fardel, Marc Le Vée
Dec 19, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Sébastien AnthérieuAndré Guillouzo
Mar 2, 2010·Nature Reviews. Drug Discovery·UNKNOWN International Transporter ConsortiumLei Zhang
Mar 17, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Steven N HartXiao-bo Zhong
Jul 14, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Marc Le VeeOlivier Fardel
Sep 25, 2010·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A KanekoK Takeda
Dec 15, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Lei GuoBaitang Ning
Feb 15, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mitchell R McGillHartmut Jaeschke
Mar 11, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sébastien AnthérieuMarie-Anne Robin
Mar 26, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Malin DarnellTommy B Andersson
Apr 2, 2011·Nature Reviews. Drug Discovery·B Kevin ParkThomas A Baillie
May 17, 2011·Biochemical Pharmacology·Marc Le VeeOlivier Fardel
Jul 6, 2011·The International Journal of Biochemistry & Cell Biology·Ruurdtje HoekstraRobert A F M Chamuleau
Jul 23, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Stéphanie MadecAnne Corlu
Sep 29, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Andreas SchulzeStephan Urban
Oct 14, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Alexandra RogueAndré Guillouzo
Oct 15, 2011·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Ugo ZanelliJ Brian Houston

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Citations

Jan 17, 2014·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ludovic Le HégaratValérie Fessard
Dec 30, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Michelle SchaeferRoderich D Süssmuth
Aug 30, 2013·Expert Opinion on Drug Metabolism & Toxicology·Guillermo Gómez-IcazbalcetaAlicia Cervantes
Oct 23, 2014·Drug Metabolism Reviews·Dong Gui HuPeter I Mackenzie
Jul 6, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kati-Sisko VellonenArto Urtti
Jan 8, 2016·Toxicology and Applied Pharmacology·Anaïs MichautBernard Fromenty
Dec 29, 2015·Biomaterials·Wen Hao TongHanry Yu
Oct 16, 2015·Biochemical Pharmacology·Abdullah MayatiOlivier Fardel
Apr 15, 2016·Nature Reviews. Gastroenterology & Hepatology·Emmanuel Thomas, T Jake Liang
Apr 12, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Andrew BaxterEmmanuel Minet
Apr 29, 2015·Cell Biology and Toxicology·Mark M T J BroekmanDirk J de Jong
Jul 16, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Marc Le VeeOlivier Fardel
Jan 29, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Natsuko TakakuraLudovic Le Hegarat
May 6, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ludovic PeyreRoger Rahmani
Jun 21, 2016·Environmental Toxicology·Simon BucherBernard Fromenty
Jun 25, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Jonathan P JacksonStephen S Ferguson
Sep 23, 2016·Viruses·Eloi R VerrierThomas F Baumert
Oct 27, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Vincent A van der MarkRuurdtje Hoekstra
Jan 27, 2015·Journal of Laboratory Automation·Salman R KhetaniBrenton R Ware
Apr 4, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Robert W YuchaMingxiang Liao
Jul 8, 2016·Experimental Biology and Medicine·Katrin ZeilingerFanny Knöspel
May 24, 2017·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Shanshan TongWeimin Cai
Sep 6, 2018·Journal of Applied Toxicology : JAT·Weijia HanZhongping Duan
Jun 21, 2018·International Journal of Toxicology·Dae-Seop ShinMyung Ae Bae
Jun 1, 2013·Hepatology International·Liesbeth CeelenMathieu Vinken
Nov 25, 2018·Pharmaceutics·Abdullah MayatiOlivier Fardel
Apr 26, 2014·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Piyanuch WongananMaria A Croyle
Jul 2, 2014·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Gustav HolmgrenJosefina Edsbagge

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