The hepatic glutathione transferases of the male little skate, Raja erinacea

Chemico-biological Interactions
G L Foureman, J R Bend

Abstract

Five cytosolic glutathione transferases were isolated from the liver of the male little skate, Raja erinacea, a marine elasmobranch. They were designated E-1 through E-5 in order of their elution from a DEAE-cellulose column with a 0 to 100 mM KCl gradient in 0.01 M Tris (pH 8.0). Each eluted peak of glutathione transferase activity, after concentration, was applied to an affinity column prepared by reaction of epoxy-activated Sepharose 6B with glutathione (GSH). Elution of the various glutathione transferases from this column with GSH resulted in the further purification of each enzyme; the major glutathione transferase, E-4 and E-1, were purified to apparent homogeneity by this procedure. Skate glutathione transferase E-4 is dimeric and the subunits are either very similar or identical in molecular weight (about 26 000 daltons). Enzymes E-2 through E-5 were acidic proteins (pI less than 7.0) and had high specific glutathione transferase activity (0.3--12 mumol/min/mg protein) with benzo[a]pyrene 4,5-oxide (BPO) as substrate, whereas the other enzyme (E-1) had low activity (0.01 mumol/min/mg) with BPO and a basic pI (greater than 9.5). Bilirubin and hematin, non-substrate ligands, bound tightly to homogeneous E-4, with dissoci...Continue Reading

References

Dec 1, 1975·European Journal of Biochemistry·K KamisakaW B Jakoby
Feb 9, 1976·Biochemical and Biophysical Research Communications·P G WislockiA H Conney
Aug 1, 1976·Archives of Biochemistry and Biophysics·W H HabigW B Jakoby
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·C F Midelfort, A H Mehler
Jun 19, 1973·Biochemical and Biophysical Research Communications·M J PabstW B Jakoby
Oct 31, 1980·Biochemical and Biophysical Research Communications·O HernandezJ R Bend
Jan 1, 1980·Hoppe-Seyler's Zeitschrift für physiologische Chemie·P Kraus
Dec 1, 1980·Archives of Biochemistry and Biophysics·T C Yeung, A S Gidari
Jun 1, 1961·The Biochemical Journal·J BoothP Sims

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Citations

Nov 1, 1990·Fish Physiology and Biochemistry·S G George, G Buchanan
Jun 1, 1987·Fish Physiology and Biochemistry·I A Nimmo
Feb 1, 1988·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·L M ValstaG S Bailey
Jan 1, 1987·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·J StenersenU Arend
Jun 26, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·W K Yuen, J W Ho
Apr 1, 1987·Environmental Health Perspectives·M O James
Sep 18, 1992·Journal of Chromatography·E Martínez-LaraJ A Bárcena
Sep 1, 1985·Archives internationales de physiologie et de biochimie·P J Dierickx

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