The hepatitis B virus MHBst167 protein is a pleiotropic transactivator mediating its effect via ubiquitous cellular transcription factors

The Journal of General Virology
W H CaselmannM Meyer

Abstract

C-terminally truncated surface proteins of hepatitis B virus (HBV) are frequently translated from genomically integrated viral sequences. They may be relevant for hepatocarcinogenesis by stimulating gene expression. First, we examined the transactivating potential of middle hepatitis B surface protein truncated at amino acid (aa) position 167 (MHBst167) on the HBV regulatory element. In transient cotransfection assays using Chang liver or HepG2 cell lines and chloramphenicol acetyltransferase (CAT) reporter constructs only the HBV enhancer I, but no other HBV regulatory elements like the X promoter, the S1 or S2 promoter or the enhancer II/core promoter could be stimulated by MHBst167. Since there is no evidence for a direct interaction of MHBst167 with DNA, we subsequently analysed whether cellular transcription factors were involved in mediating transactivation. This was tested both with isolated transcription-factor-binding sites and in the natural context of viral and cellular promoter elements. Deletion analysis and electrophoretic mobility shift assays revealed that Sp1, AP1 and NF-kappa B can mediate transactivation by MHBst167. No involvement of CREB, NF1 or the liver-specific factor C/EBP was found. These data indicate...Continue Reading

Citations

Feb 2, 2010·Pathologie-biologie·F V ChisariS F Wieland
Mar 15, 2006·Critical Reviews in Clinical Laboratory Sciences·Chi-Hang WongMark Feitelson
Nov 15, 2000·Critical Reviews in Clinical Laboratory Sciences·C Rabe, W H Caselmann
Sep 25, 2019·International Journal of Molecular Sciences·Daniela Bender, Eberhard Hildt

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