The hepatitis C virus 3'-untranslated region or a poly(A) tract promote efficient translation subsequent to the initiation phase

Nucleic Acids Research
Shelton BradrickMatthias Gromeier

Abstract

Enhancement of eukaryotic messenger RNA (mRNA) translation initiation by the 3' poly(A) tail is mediated through interaction of poly(A)-binding protein with eukaryotic initiation factor (eIF) 4G, bridging the 5' terminal cap structure. In contrast to cellular mRNA, translation of the uncapped, non-polyadenylated hepatitis C virus (HCV) genome occurs independently of eIF4G and a role for 3'-untranslated sequences in modifying HCV gene expression is controversial. Utilizing cell-based and in vitro translation assays, we show that the HCV 3'-untranslated region (UTR) or a 3' poly(A) tract of sufficient length interchangeably stimulate translation dependent upon the HCV internal ribosomal entry site (IRES). However, in contrast to cap-dependent translation, the rate of initiation at the HCV IRES was unaffected by 3'-untranslated sequences. Analysis of post-initiation events revealed that the 3' poly(A) tract and HCV 3'-UTR improve translation efficiency by enabling termination and possibly ribosome recycling for successive rounds of translation.

References

Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·M YanagiJ Bukh
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M YanagiJ Bukh
Mar 10, 2001·RNA·J S KieftJ A Doudna
Apr 3, 2001·Nature Reviews. Molecular Cell Biology·C J WiluszS W Peltz
May 25, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Anton P McCaffreyMark A Kay
Oct 17, 2002·The Journal of Biological Chemistry·Naoyuki UchidaToshiaki Katada
Jan 7, 2003·The EMBO Journal·Lev KisselevLudmila Frolova
May 15, 2003·JAMA : the Journal of the American Medical Association·Steven L Flamm
Dec 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Elena DobrikovaMatthias Gromeier
Oct 28, 2004·Cell·Geoff A Otto, Joseph D Puglisi
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hong JiJennifer A Doudna

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Citations

Oct 14, 2006·Virus Genes·Michèle BrocardKatherine M Kean
Oct 3, 2013·Nature Communications·Ka-Wei TangErik Larsson
Oct 16, 2012·Nucleic Acids Research·Cristina Romero-LópezAlfredo Berzal-Herranz
Sep 21, 2013·Nucleic Acids Research·Cristina Romero-LópezAlfredo Berzal-Herranz
May 25, 2007·Journal of Virology·Timothy L TellinghuisenCharles M Rice
Jan 29, 2011·Gastroenterology Research and Practice·Helene Minyi Liu, Michael Gale
Jul 17, 2009·RNA·Cristina Romero-López, Alfredo Berzal-Herranz
Oct 26, 2013·Archives of Virology·K Dominik Conrad, Michael Niepmann
May 21, 2011·Cellular and Molecular Life Sciences : CMLS·Cristina Romero-López, Alfredo Berzal-Herranz
Feb 10, 2012·Current Opinion in Virology·Lucas C Reineke, Richard E Lloyd
Oct 31, 2012·Science China. Life Sciences·Xue ZhaoHaitao Zhao
Jul 28, 2009·Biochimica Et Biophysica Acta·Laurent BalvayThéophile Ohlmann
May 15, 2009·Biochimica Et Biophysica Acta·Michael Niepmann
Jul 17, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Jae-Ku OemQiang Liu
Nov 21, 2008·The EMBO Journal·Jura Inga HenkeMichael Niepmann
Jul 13, 2011·Liver International : Official Journal of the International Association for the Study of the Liver·Brett Hoffman, Qiang Liu
Aug 29, 2013·Reviews in Medical Virology·Cristina Romero-López, Alfredo Berzal-Herranz
Apr 16, 2015·Journal of Digestive Diseases·Ismail M ElshaffeiGeorge Y Wu
Aug 4, 2015·Biochemical and Biophysical Research Communications·Brett HoffmanQiang Liu
Apr 21, 2007·Virology·Miguel Rodríguez PulidoEncarnación Martínez-Salas
May 9, 2007·Gastroenterology·Jean-Michel PawlotskyJohn G McHutchison
Nov 18, 2016·Cellular and Molecular Life Sciences : CMLS·Ilya M TereninIvan N Shatsky

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Methods Mentioned

BETA
PCR
electrophoresis
transfection
density gradient fractionation
density gradient centrifugation
GTPase

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