PMID: 9557660Apr 29, 1998Paper

The herpes simplex virus type 1 U(L)17 gene encodes virion tegument proteins that are required for cleavage and packaging of viral DNA

Journal of Virology
B SalmonJ D Baines

Abstract

Previous studies have suggested that the U(L)17 gene of herpes simplex virus type 1 (HSV-1) is essential for virus replication. In this study, viral mutants incorporating either a lacZ expression cassette in place of 1,490 bp of the 2,109-bp U(L)17 open reading frame [HSV-1(deltaU(L)17)] or a DNA oligomer containing an in-frame stop codon inserted 778 bp from the 5' end of the U(L)17 open reading frame [HSV-1(U(L)17-stop)] were plaque purified on engineered cell lines containing the U(L)17 gene. A virus derived from HSV-1(U(L)17-stop) but containing a restored U(L)17 gene was also constructed and was designated HSV-1(U(L)17-restored). The latter virus formed plaques and cleaved genomic viral DNA in a manner indistinguishable from wild-type virus. Neither HSV-1(deltaU(L)17) nor HSV-1(U(L)17-stop) formed plaques or produced infectious progeny when propagated on noncomplementing Vero cells. Furthermore, genomic end-specific restriction fragments were not detected in DNA purified from noncomplementing cells infected with HSV-1(deltaU(L)17) or HSV-1(U(L)17-stop), whereas end-specific fragments were readily detected when the viruses were propagated on complementing cells. Electron micrographs of thin sections of cells infected with H...Continue Reading

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Citations

Oct 24, 2008·Journal of Virology·Shelley K CockrellFred L Homa
Mar 17, 2009·Veterinary Research·Najat ChbabJean-François Vautherot
Jun 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wouter H RoosGijs J L Wuite
Apr 16, 2011·Nature Reviews. Microbiology·David C Johnson, Joel D Baines
Sep 2, 2011·Journal of Virology·Mindy LeelawongGregory A Smith
Dec 10, 1999·Journal of Virology·R J RollerD DeSalvo
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Lisa E PomeranzChristoph J Hengartner
Jul 24, 2014·Nucleic Acids Research·Udom Sae-UengAlex Evilevitch
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D G WolfE S Mocarski
Jun 14, 2003·Journal of Virology·Susan L BjerkeRichard J Roller
May 23, 2007·Pigment Cell Research·Jón Hallsteinn HallssonEiríkur Steingrímsson
Oct 30, 2009·Journal of Virology·Vladislav A LobanovSylvia van Drunen Littel-van den Hurk
Sep 15, 2007·Journal of Virology·David G Meckes, John W Wills
Aug 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Kui Yang, Joel D Baines
Aug 27, 2015·Virology Journal·Robyn N HallTimothy J Mahony
Jul 7, 2017·Journal of Virology·Ryosuke KobayashiYasushi Kawaguchi

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