The heterogeneity of target recognition by lymphokine-activated killer precursor cells

Japanese Journal of Cancer Research : Gann
Y IbayashiK Hashi

Abstract

Lymphokine-activated killer (LAK) cells were generated from peripheral blood lymphocytes (PBL) that were depleted of mature cytotoxic natural killer (NK) cells. PBL NK activity was abolished by pretreatment of effector cells with the toxic lysosomotropic agent L-leucine methyl ester (LME) or by depletion of effector cells by K562 monolayer absorption (MA). Both treatments markedly reduced the proportion of cells expressing NK-associated markers such as CD 16 (Leu 11b, B73.1), Leu 7, and NKH-1 (Leu 19), whereas these treatments had minimal effects on cells expressing T cell markers (CD 3, CD 4, and CD 8). LME and MA also drastically decreased the proportion of K562 target-binding lymphocytes. LAK activity against NK-sensitive and NK-resistant targets can be generated from the NK cell-depleted PBL by incubation with interleukin-2. Peak LAK activity generated from MA-treated PBL was later than the peak of LAK activity generated from either untreated or LME-treated PBL. Although MA of PBL on NK-resistant S4 sarcoma targets had little effect on NK activity, LAK activity against both K562 and S4 targets was reduced. These results suggest that there are at least three LAK precursor subpopulations in PBL: mature NK cells that can bind ...Continue Reading

References

Dec 1, 1987·Cellular Immunology·Y IbayashiS H Golub
Jul 15, 1987·International Journal of Cancer. Journal International Du Cancer·I AndoR F Irie
Jul 29, 1983·Journal of Immunological Methods·L M Karavodin, S H Golub
Dec 1, 1983·European Journal of Immunology·M L ToribioM López-Botet
Jul 15, 1983·International Journal of Cancer. Journal International Du Cancer·J A WerkmeisterJ C Roder

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.

Related Papers

The Journal of Immunology : Official Journal of the American Association of Immunologists
H Shau, S H Golub
Hepatology : Official Journal of the American Association for the Study of Liver Diseases
K HataT L Whiteside
© 2021 Meta ULC. All rights reserved