The heterotetrameric architecture of the epithelial sodium channel (ENaC)

The EMBO Journal
D FirsovL Schild

Abstract

The epithelial sodium channel (ENaC) is a key element for the maintenance of sodium balance and the regulation of blood pressure. Three homologous ENaC subunits (alpha, beta and gamma) assemble to form a highly Na+-selective channel. However, the subunit stoichiometry of ENaC has not yet been solved. Quantitative analysis of cell surface expression of ENaC alpha, beta and gamma subunits shows that they assemble according to a fixed stoichiometry, with alpha ENaC as the most abundant subunit. Functional assays based on differential sensitivities to channel blockers elicited by mutations tagging each alpha, beta and gamma subunit are consistent with a four subunit stoichiometry composed of two alpha, one beta and one gamma. Expression of concatameric cDNA constructs made of different combinations of ENaC subunits confirmed the four subunit channel stoichiometry and showed that the arrangement of the subunits around the channel pore consists of two alpha subunits separated by beta and gamma subunits.

References

Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·L SchildB C Rossier
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·D FirsovB C Rossier
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J García-AñoverosD P Corey
Mar 13, 1997·Nature·R WaldmannM Lazdunski
Jan 1, 1997·Annual Review of Physiology·N Tavernarakis, M Driscoll
Jun 1, 1997·The Journal of General Physiology·C M McNicholas, C M Canessa

❮ Previous
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Citations

Sep 3, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·I Mano, M Driscoll
Jun 30, 2005·The Journal of Membrane Biology·M M ReddyP M Quinton
Jan 28, 2006·The Journal of Membrane Biology·N SallehS R Milligan
Sep 18, 2007·Pflügers Archiv : European journal of physiology·Martin Fronius, Wolfgang G Clauss
Mar 12, 2009·Pflügers Archiv : European journal of physiology·Johannes Loffing, Christoph Korbmacher
Nov 18, 2006·Cancer Metastasis Reviews·Jamie L FiskeRobert A Sikes
May 5, 2006·Current Hypertension Reports·Carolyn A Ecelbarger, Swasti Tiwari
Nov 28, 2012·Journal of Physiology and Biochemistry·G G RobinsG I Sandle
Jan 19, 2000·Steroids·F M Rogerson, P J Fuller
Jul 31, 2003·Progress in Biophysics and Molecular Biology·Albertino BigianiFrancesca Fieni
Feb 9, 2000·Pharmacology & Therapeutics·M K Agarwal, M Mirshahi
Aug 28, 1998·Current Opinion in Cell Biology·J D Horisberger
Sep 12, 2001·Trends in Endocrinology and Metabolism : TEM·D Pearce
Nov 16, 2012·Chemical Reviews·Mark A ZaydmanJianmin Cui
May 31, 2011·Acta Pharmacologica Sinica·Yan SunZhi-ren Zhang
Oct 3, 1999·Kidney International·G Gamba
Mar 18, 2000·Kidney International·O StaubD Rotin
Apr 12, 2000·Kidney International·P J FullerF E Brennan
Apr 12, 2000·Kidney International·O Bonny, E Hummler
Jan 20, 2004·Advances in Renal Replacement Therapy·Pauline A Swift, Graham A Macgregor
Jul 22, 2011·The Journal of Biological Chemistry·Andrew P StewartJ Michael Edwardson
Aug 1, 2007·The Journal of Biological Chemistry·Ossama B KashlanThomas R Kleyman
Aug 21, 2008·The Journal of Biological Chemistry·Michael B ButterworthJohn P Johnson
Feb 24, 2000·The Journal of Experimental Medicine·A de Baey, A Lanzavecchia
Jul 10, 1999·The Journal of General Physiology·S KellenbergerL Schild
Dec 1, 2000·The Journal of General Physiology·P M SnyderD R Olson
Nov 28, 2001·The Journal of General Physiology·S KellenbergerL Schild
Oct 3, 2002·The Journal of General Physiology·Ping Zhang, Cecilia M Canessa
Sep 29, 2004·The Journal of General Physiology·Diego Alvarez de la RosaCecilia M Canessa
Dec 30, 2004·The Journal of General Physiology·Michael B ButterworthRaymond A Frizzell
Jun 15, 2007·The Journal of General Physiology·Arun Anantharam, Lawrence G Palmer
Dec 8, 1998·Molecular Biology of the Cell·L MastroberardinoF Verrey
Aug 21, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ingrid M CurrilJeffrey L Spees
Sep 16, 2000·Current Opinion in Nephrology and Hypertension·D Rotin
Aug 19, 2007·Current Opinion in Nephrology and Hypertension·Rebecca P HugheyThomas R Kleyman
Feb 12, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sergei Sukharev, David P Corey
Jun 29, 2000·Annual Review of Biochemistry·D L Garbers, S K Dubois

❮ Previous
Next ❯

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