The hfq gene is required for stress resistance and full virulence of Burkholderia cepacia to the nematode Caenorhabditis elegans

Microbiology
Sílvia A SousaJorge H Leitão

Abstract

The Burkholderia cepacia complex (Bcc) emerged as problematic opportunistic pathogens to cystic fibrosis (CF) patients. Although several virulence factors have been identified in Bcc, the knowledge of their relative contribution to Bcc pathogenicity remains scarce. In this work, we describe the identification and characterization of a B. cepacia IST408 mutant containing a disruption in the hfq gene. In other bacteria, Hfq is a global regulator of metabolism, acting as an RNA chaperone involved in the riboregulation of target mRNAs by small regulatory non-coding RNAs (sRNAs). The B. cepacia Hfq protein was overproduced as a histidine-tagged derivative, and we show evidence that the protein forms hexamers and binds sRNAs. When provided in trans, the B. cepacia IST408 hfq gene complemented the Escherichia coli hfq mutant strain GS081. Our results also show that the B. cepacia hfq mutant is more susceptible to stress conditions mimicking those faced by Bcc bacteria when infecting the CF host. In addition, the B. cepacia hfq mutant and two hfq mutants derived from B. dolosa and B. ambifaria clinical isolates also exhibited a reduced ability to colonize and kill the nematode Caenorhabditis elegans, used as an infection model. These d...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
May 1, 1991·Journal of Bacteriology·P HübnerT A Bickle
May 1, 1974·Genetics·S Brenner
Aug 10, 1968·Nature·M T Franze de FernandezJ T August
Feb 1, 1984·The Journal of Pediatrics·A IslesH Levison
Jan 1, 1982·The Journal of Cell Biology·T M Roberts, S Ward
Mar 17, 1999·Methods : a Companion to Methods in Enzymology·V Jackson
Jul 19, 2000·Current Biology : CB·B K Pannone, S L Wolin
Jan 24, 2002·Molecular Cell·Aixia ZhangGisela Storz
Mar 16, 2002·Infection and Immunity·Mladen TomichChristian D Mohr
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
Jun 7, 2003·Journal of Clinical Microbiology·Tom CoenyeEshwar Mahenthiralingam
Jul 11, 2003·Nucleic Acids Research·Claude SauterDietrich Suck
Oct 3, 2003·Microbial Pathogenesis·Elisabeth SonnleitnerUdo Bläsi
Nov 26, 2003·Biochemical and Biophysical Research Communications·Leonilde M MoreiraIsabel Sá-Correia
Mar 11, 2004·Molecular Microbiology·Poul Valentin-HansenChristina Udesen
Jul 1, 2004·Molecular Microbiology·Yanpeng DingMatthew K Waldor
Jan 12, 2005·Nature Reviews. Microbiology·Eshwar MahenthiralingamJoanna B Goldberg
May 26, 2005·Trends in Genetics : TIG·Susan Gottesman
Jul 27, 2005·FEMS Microbiology Letters·Silvia T CardonaMiguel A Valvano
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Terence I MoyFrederick M Ausubel
Dec 14, 2006·Molecular Microbiology·Alexandra SittkaJörg Vogel
Dec 23, 2006·Biochemical and Biophysical Research Communications·Sílvia A SousaJorge H Leitão
Mar 31, 2007·Current Opinion in Microbiology·Richard G Brennan, Todd M Link
Oct 16, 2007·Nucleic Acids Research·Victor M MarkowitzNikos C Kyrpides
Oct 17, 2007·FEMS Microbiology Letters·Tom CoenyeDavid W Ussery
Jan 26, 2008·Journal of Applied Microbiology·E MahenthiralingamC G Dowson
Dec 17, 2008·Journal of Medical Microbiology·Siobhán McClean, Máire Callaghan
Feb 3, 2009·Journal of Bacteriology·Dipak DuttaPradeep Parrack

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Citations

Jun 5, 2013·Infection and Immunity·Sargurunathan SubashchandraboseMartha H Mulks
Jun 5, 2013·Journal of Bacteriology·Christian G RamosJorge H Leitão
Jun 19, 2013·BMC Microbiology·Randy J HempelTerrence L Stull
Nov 3, 2012·PloS One·Patricio SobreroClaudio Valverde
Apr 15, 2010·Applied Microbiology and Biotechnology·Jorge H LeitãoLeonilde M Moreira
May 11, 2013·International Journal of Medical Microbiology : IJMM·Chee-Hock HoeThean-Hock Tang
Jan 19, 2010·Current Opinion in Microbiology·Yanjie Chao, Jörg Vogel
Dec 20, 2015·Future Microbiology·Joana R FelicianoJorge H Leitão
Mar 7, 2014·Environmental Microbiology·Mustafa FazliTim Tolker-Nielsen
Jun 29, 2015·Research in Microbiology·Guang YangHongbin Song
Sep 3, 2010·International Journal of Microbiology·Sílvia A SousaJorge H Leitão
Sep 17, 2015·Environmental Microbiology·Alejandro Arce-RodríguezVíctor de Lorenzo
Mar 23, 2012·Critical Reviews in Microbiology·Patricio Sobrero, Claudio Valverde
Apr 6, 2018·Clinical Microbiology Reviews·Maria MaganaGeorge P Tegos
Oct 30, 2018·The Plant Pathology Journal·Jieun KimYoung-Su Seo
May 2, 2021·Applied and Environmental Microbiology·Sara C GomesLeonilde M Moreira
Jul 30, 2021·Frontiers in Cellular and Infection Microbiology·Louise Djapgne, Amanda G Oglesby

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