The hierarchically organized splitting of chromosome bands into sub-bands analyzed by multicolor banding (MCB)

Cytogenetic and Genome Research
H LehrerThomas Liehr

Abstract

To clarify the nature of chromosome sub-bands in more detail, the multicolor banding (MCB) probe-set for chromosome 5 was hybridized to normal metaphase spreads of GTG band levels at approximately 850, approximately 550, approximately 400 and approximately 300. It could be observed that as the chromosomes became shorter, more of the initial 39 MCB pseudo-colors disappeared, ending with 18 MCB pseudo-colored bands at the approximately 300-band level. The hierarchically organized splitting of bands into sub-bands was analyzed by comparing the disappearance or appearance of pseudo-color bands of the four different band levels. The regions to split first are telomere-near, centromere-near and in 5q23-->q31, followed by 5p15, 5p14, and all GTG dark bands in 5q apart from 5q12 and 5q32 and finalized by sub-band building in 5p15.2, 5q21.2-->q21.3, 5q23.1 and 5q34. The direction of band splitting towards the centromere or the telomere could be assigned to each band separately. Pseudo-colors assigned to GTG-light bands were resistant to band splitting. These observations are in concordance with the recently proposed concept of chromosome region-specific protein swelling.

Citations

Oct 31, 2006·Chromosoma·Maria CostantiniGiorgio Bernardi
Jan 28, 2009·Molecular Cytogenetics·Nadezda KosyakovaHeike Nelle
Feb 8, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anja WeiseNadezda Kosyakova
Oct 6, 2010·Molecular Cytogenetics·Walid Al AchkarThomas Liehr
Sep 7, 2006·Cytogenetic and Genome Research·T LiehrA Weise
Apr 23, 2008·Current Protocols in Human Genetics·Joan H M Knoll, Peter Lichter
Feb 17, 2019·International Journal of Molecular Sciences·Thomas Liehr

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