The high-affinity phosphate transporter Pst is a virulence factor for Proteus mirabilis during complicated urinary tract infection

FEMS Immunology and Medical Microbiology
S M JacobsenHarry L T Mobley

Abstract

Proteus mirabilis is a ubiquitous bacterium associated with complicated urinary tract infection (UTI). Mutagenesis studies of the wild-type strain HI4320 in the CBA mouse model of ascending UTIs have identified attenuated mutants with transposon insertions in genes encoding the high-affinity phosphate transporter Pst (pstS, pstA). The transcription of the pst operon (pstSCAB-phoU) and other members of the phosphate regulon of Escherichia coli, including alkaline phosphatase (AP), are regulated by the two-component regulatory system PhoBR and are repressed until times of phosphate starvation. This normal suppression was relieved in pstS::Tn5 and pstA::Tn5 mutants, which constitutively produced AP regardless of growth conditions. No significant growth defects were observed in vitro for the pst mutants during the independent culture or coculture studies in rich broth, phosphate-limiting minimal salts medium, or human urine. Mutants complemented with the complete pst operon repressed AP synthesis in vitro and colonized the mouse bladder in numbers comparable to the wild-type strain HI4320. Therefore, the Pst transport system imparts a significant in vivo advantage to wild-type P. mirabilis that is not required for in vitro growth. ...Continue Reading

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Citations

Jun 29, 2010·Applied and Environmental Microbiology·Quan WangLei Wang
Jan 23, 2016·Archives of Microbiology·Fernanda Nogales da Costa VasconcelosBeny Spira
Jun 28, 2011·Veterinary Microbiology·Sébastien CrépinJosée Harel
Aug 23, 2008·Journal of Medical Microbiology·Stephanie D HimpslHarry L T Mobley
Mar 24, 2016·Molecular Plant-microbe Interactions : MPMI·Tiyakhon ChatnaparatSteven E Lindow
Feb 22, 2018·Proteomics·Laurence Don Wai LuuRuiting Lan
Aug 21, 2016·Applied and Environmental Microbiology·L HudekL Bräu
Apr 11, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Anne BernaFrançois Bernier
Sep 22, 2017·Veterinary Research·Gokhlesh KumarMansour El-Matbouli
Mar 7, 2021·International Journal of Molecular Sciences·Elena PorzioGiuseppe Manco
Feb 10, 2018·EcoSal Plus·Chelsie E ArmbrusterMelanie M Pearson
Nov 7, 2015·Microbiology Spectrum·Jessica N Schaffer, Melanie M Pearson

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