The high concentration of Arg213-->Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities

The Biochemical Journal
Steen V PetersenJ J Enghild

Abstract

The C-terminal region of EC-SOD (extracellular superoxide dismutase) mediates the binding to both heparin/heparan sulphate and type I collagen. A mutation (Arg213-->Gly; R213G) within this extracellular matrix-binding region has recently been implicated in the development of heart disease. This relatively common mutation affects the heparin affinity, and the concentration of EC-SOD in the plasma of R213G homozygous individuals is increased 10- to 30-fold. In the present study we confirm, using R213G EC-SOD purified from a homozygous individual, that the heparin affinity is reduced. Significantly, the collagen affinity of the R213G EC-SOD variant was similarly affected and both the heparin and collagen affinities were reduced by 12-fold. Structural analysis of synthetic extracellular matrix-binding regions suggests that the mutation alters the secondary structure. We conclude that the increased concentration of EC-SOD in the plasma of R213G carriers is caused by a reduction in both heparin and collagen affinities.

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Citations

May 15, 2010·Applied Biochemistry and Biotechnology·Young-Jin SonHan Choe
Apr 4, 2008·The Journal of Biological Chemistry·Steen V PetersenJan J Enghild
Jan 10, 2006·American Journal of Respiratory and Critical Care Medicine·Klaus JuulBørge G Nordestgaard
Jan 19, 2008·Translational Research : the Journal of Laboratory and Clinical Medicine·Zhenyu QinNeal L Weintraub
May 31, 2011·Free Radical Biology & Medicine·Elizabeth A ReganVuokko L Kinnula
May 3, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Steen V Petersen, Jan J Enghild
Jan 24, 2009·Genesis : the Journal of Genetics and Development·Yani ZouTing-Ting Huang
Dec 26, 2018·Antioxidants & Redox Signaling·Victor TsengC Michael Hart
Nov 17, 2010·Molecular Biology Reports·Jian ZhaoJian-Wen Liu
Jan 17, 2015·Journal of Applied Physiology·Kathryn M PateElizabeth A Regan
Sep 8, 2017·JCI Insight·Rohit GauravRussell P Bowler
Nov 15, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Nguyen Hoai NguyenCuong Thach Nguyen
Jan 22, 2021·Free Radical Biology & Medicine·Cecilie L MatthiesenSteen V Petersen

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