The high copper tolerance of Candida albicans is mediated by a P-type ATPase

Proceedings of the National Academy of Sciences of the United States of America
Ziva WeissmanD Kornitzer

Abstract

The pathogenic yeast Candida albicans has higher resistance than the baker's yeast Saccharomyces cerevisiae to elevated concentrations of copper. To understand the basis of this differential resistance, we performed a functional screen for C. albicans genes involved in copper detoxification. Here, we report the isolation of two such genes: a metallothionein, CaCUP1, and a copper-transporting P-type ATPase, CaCRP1. Both genes are induced by extracellular copper. Gene disruptions indicated that the copper extrusion pump is responsible for the unusual resistance of C. albicans to copper, whereas the metallothionein is responsible for the residual copper resistance of the Cacrp1Delta mutant. We show further that under acidic and anaerobic conditions, such as prevail in the natural niche of C. albicans, the digestive tract of animals, CaCRP1 function becomes essential for survival in the presence of even very low copper concentrations. These observations suggest that copper in the gastrointestinal tract may present a toxic challenge to which enteric organisms had to adapt.

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Related Concepts

AF193508
AF193509
DNA-dependent ATPase
Candida albicans
Copper
Metallothionein IIA
Saccharomyces cerevisiae
Gene Expression Regulation, Fungal
Gene Expression Regulation, Enzymologic
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