The high-mobility group A-type protein CarD of the bacterium Myxococcus xanthus as a transcription factor for several distinct vegetative genes

Genetics
Marisa Galbis-MartínezF J Murillo

Abstract

CarD is the only reported prokaryotic protein showing structural and functional features typical of eukaryotic high-mobility group A transcription factors. In prokaryotes, proteins similar to CarD appear to be confined primarily to myxobacteria. In Myxococcus xanthus, CarD has been previously shown to act as a positive element in two different regulatory networks: one for light-induced synthesis of carotenoids and the other for starvation-induced fruiting body formation. We have now tested the effect of a loss-of-function mutation in the carD gene (carD1) on the expression of a random collection of lacZ-tagged genes, which are normally expressed in the dark during vegetative growth in rich medium. Our results indicate that CarD plays a significant role in the transcriptional regulation of various indicated genes. The carD1 mutation downregulates some genes and upregulates others. Also reported here is the isolation of several mutations that suppress the strong effect of carD1 on the expression of a particular vegetative gene. One of them (sud-2) also suppresses the effect of carD1 on other vegetative genes and on fruiting-body formation. Thus, CarD and the sud-2 gene product appear to participate in a single mechanism, which un...Continue Reading

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Citations

Aug 11, 2005·Journal of Industrial Microbiology & Biotechnology·Hideaki TakanoKenji Ueda
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Francisco García-HerasMontserrat Elías-Arnanz
Jan 12, 2010·PloS One·Carey LambertR Elizabeth Sockett
May 11, 2005·Molecular Microbiology·Aurelio Moraleda-MuñozJosé Muñoz-Dorado
Mar 4, 2008·Microbiology·Lilian Galbis-MartínezMarta Fontes
Jun 22, 2010·FEMS Microbiology Reviews·Montserrat Elías-ArnanzFrancisco J Murillo
Jul 10, 2018·Emerging Microbes & Infections·Tong ChenX Frank Yang
Sep 8, 2017·Frontiers in Microbiology·Lei PanGraciela L Lorca

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