The highly charged region of plant beta-type phosphatidylinositol 4-kinase is involved in membrane targeting and phospholipid binding.

Plant Molecular Biology
Ying LouHong-Wei Xue

Abstract

In Arabidopsis thaliana and Oryza sativa, two types of PI 4-kinase (PI4Ks) have been isolated and functionally characterized. The alpha-type PI4Ks (approximately 220 kDa) contain a PH domain, which is lacking in beta-type PI4Ks (approximately 120 kDa). Beta-type PI4Ks, exemplified by Arabidopsis AtPI4Kbeta and rice OsPI4K2, contain a highly charged repetitive segment designated PPC (Plant PI4K Charged) region, which is an unique domain only found in plant beta-type PI4Ks at present. The PPC region has a length of approximately 300 amino acids and harboring 11 (AtPI4Kbeta) and 14 (OsPI4K2) repeats, respectively, of a 20-aa motif. Studies employing a modified yeast-based "Sequence of Membrane-Targeting Detection" system demonstrate that the PPC(OsPI4K2) region, as well as the former 8 and latter 6 repetitive motifs within the PPC region, are able to target fusion proteins to the plasma membrane. Further detection on the transiently expressed GFP fusion proteins in onion epidermal cells showed that the PPC(OsPI4K2) region alone, as well as the region containing repetitive motifs 1-8, was able to direct GFP to the plasma membrane, while the regions containing less repetitive motifs, i.e. 6, 4, 2 or single motif(s) led to predominan...Continue Reading

References

Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G Ginsburg, A R Kimmel
Oct 20, 1987·Biochemistry·G EndemannL C Cantley
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Sep 1, 1995·The Plant Journal : for Cell and Molecular Biology·J ShiM K Bhattacharyya
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·T HirayamaK Shinozaki
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·Z Hong, D P Verma
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·P WeltersM J Chrispeels
Dec 24, 1997·Current Topics in Microbiology and Immunology·M A Lemmon, K M Ferguson
Aug 26, 1998·The Journal of Biological Chemistry·J M StevensonW F Boss
Oct 1, 1998·The Plant Journal : for Cell and Molecular Biology·K MikamiK Shinozaki
Dec 5, 1998·Biochimica Et Biophysica Acta·K A HinchliffeR F Irvine
Mar 20, 1999·The Journal of Biological Chemistry·L E Rameh, L C Cantley
May 14, 1999·Trends in Cell Biology·M G Roth
Mar 29, 2001·Nature Structural Biology·P C Driscoll
May 4, 2001·Trends in Plant Science·T Munnik
Dec 14, 2002·Plant Physiology·Christian SohlenkampMichael K Udvardi
Mar 1, 1956·Canadian Journal of Biochemistry and Physiology·L E HOKIN, M R HOKIN

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Citations

Mar 9, 2013·Plant Cell Reports·Martin JandaEric Ruelland
Mar 10, 2012·The New Phytologist·Shiv D Kale
Aug 18, 2012·Plant Signaling & Behavior·Elise DelageJuliette Puyaubert
Nov 26, 2008·Current Opinion in Plant Biology·Julie M Thole, Erik Nielsen
Apr 17, 2018·Journal of Integrative Plant Biology·Hong-Yan Yao, Hong-Wei Xue
Jun 26, 2009·The Biochemical Journal·Hong-Wei XueYu Mei

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