The highly conserved COOH terminus of troponin I forms a Ca2+-modulated allosteric domain in the troponin complex

Biochemistry
J P JinA Chen

Abstract

The primary structure of the COOH-terminal region of troponin I (TnI) is highly conserved among the cardiac, slow, and fast skeletal muscle TnI isoforms and across species. Although no binding site for the other thin filament proteins is found at the COOH terminus of TnI, truncations of the last 19-23 amino acid residues reduce the activity of TnI in the inhibition of actomyosin ATPase and result in cardiac muscle malfunction. We have developed a specific monoclonal antibody (mAb), TnI-1, against the conserved COOH terminus of TnI. Using this mAb, isolation of the troponin complex by immunoaffinity chromatography from muscle homogenate and immunofluorescence microscopic staining of myofibrils indicate that the COOH terminus of TnI forms an exposed structure in the muscle thin filament. Binding of this mAb to the COOH terminus of cardiac TnI induced extensive conformational changes in the protein, suggesting an allosteric role of this region in the functional integrity of troponin. In the absence of Ca2+, the binding of troponin C and troponin T to TnI had very little effect on the conformation of the COOH terminus of TnI as indicated by the unaffected mAb affinity for the TnI-1 epitope. However, Ca2+ significantly increased the...Continue Reading

References

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Citations

Apr 15, 2009·Journal of Molecular Evolution·Stephen M Chong, J-P Jin
Dec 12, 2013·Archives of Biochemistry and Biophysics·Shirin AkhterJ-P Jin
Jan 31, 2007·Biochemistry·Brandon J BiesiadeckiJian-Ping Jin
Oct 5, 2010·The Journal of Biological Chemistry·Julie Mouannes KozailiLarry S Tobacman
Oct 5, 2010·The Journal of Biological Chemistry·Devanand Kowlessur, Larry S Tobacman
Oct 16, 2012·The Journal of Biological Chemistry·Devanand Kowlessur, Larry S Tobacman
Jun 27, 2008·American Journal of Physiology. Cell Physiology·Lars LarssonJ-P Jin
Sep 30, 2005·American Journal of Physiology. Cell Physiology·Marco A BrottoJian-Ping Jin
Nov 17, 2006·American Journal of Physiology. Cell Physiology·Zhi Bin YuJian-Ping Jin
Dec 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Shirin AkhterJ-P Jin
Apr 27, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Han-Zhong Feng, J-P Jin
Apr 15, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Jianfeng DuXu-Pei Huang
Jan 19, 2013·Circulation Research·R John SolaroTomoyoshi Kobayashi
Jan 9, 2010·Bioscience, Biotechnology, and Biochemistry·Michael W Lassalle
Dec 26, 2012·The Journal of Physiology·Guillaume RenaudLars Larsson
Feb 17, 2007·Archives of Biochemistry and Biophysics·Zhi-Bin Yu, Jiang-Ping Jin
Mar 15, 2016·Archives of Biochemistry and Biophysics·Nancy L Meyer, P Bryant Chase
Sep 17, 2004·Archives of Biochemistry and Biophysics·Thomas M NosekJian-Ping Jin
Feb 14, 2006·Journal of Molecular Biology·Alnoor PiraniWilliam Lehman
Nov 4, 2015·Journal of Molecular and Cellular Cardiology·Juan-Juan ShengJ-P Jin
Feb 26, 2013·Journal of Veterinary Cardiology : the Official Journal of the European Society of Veterinary Cardiology·Mohamed TharwatSébastien Buczinski
Jan 9, 2018·Journal of Strength and Conditioning Research·Jenney LiuMoh H Malek
Jun 23, 2018·Human Molecular Genetics·Michael D FoxKevin A Strauss
Dec 22, 2004·The Journal of Biological Chemistry·John C BarbatoJian-Ping Jin
Jan 31, 2014·American Journal of Physiology. Cell Physiology·Rong LiuJ-P Jin

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