PMID: 8943016Nov 26, 1996Paper

The highly conserved skb1 gene encodes a protein that interacts with Shk1, a fission yeast Ste20/PAK homolog

Proceedings of the National Academy of Sciences of the United States of America
M GilbrethStevan Marcus

Abstract

The Shk1 protein kinase, a homolog of Saccharomyces cerevisiae Ste20 and mammalian p21Cdc42/Rac-activated kinases, is an essential component of a Ras- and Cdc42-dependent signaling cascade required for cell viability, normal morphology, and mitogen-activated protein kinase-mediated sexual responses in the fission yeast, Schizosaccharomyces pombe. To identify S. pombe proteins that modulate or mediate Shk1 functions, we conducted a two-hybrid screen for Shk1-interacting proteins. One of the genes identified as a result of this screen was skb1. We show that Skb1 interacts with a region of the N-terminal regulatory domain of Shk1 distinct from that to which Cdc42 binds, and that Shk1, Cdc42, and Skb1 are able to form a ternary complex in vivo. S.pombe cells carrying an skb1 null mutation are less elongate in morphology than wild-type cells and exhibit a moderate growth defect. The morphology defect of the skb1 deletion mutant is suppressed by overexpression of Shk1. Overexpression of Skb1 causes wild-type S. pombe cells to become hyperelongated. Additional genetic analyses described herein suggest that Skb1 is a component of the morphology control branch of the Ras signaling cascade in S. pombe and that it positively modulates Shk...Continue Reading

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Citations

Mar 6, 1999·Current Opinion in Microbiology·F Verde
Apr 26, 2013·Molecular Biology of the Cell·Lin Deng, James B Moseley
Jul 3, 2007·Eukaryotic Cell·Deborah A PasternackLaurie K Read
Jul 25, 2000·Biochemical and Biophysical Research Communications·J H LeeS Pestka
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M GilbrethS Marcus
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·A FujitaS Tanaka
Jul 11, 2014·Molecular Biology of the Cell·Lin DengJames B Moseley
Mar 27, 2010·Fungal Genetics and Biology : FG & B·Ingo BauerGerald Brosch
Sep 29, 2006·Pharmacology & Therapeutics·Christopher D KrauseSidney Pestka
Jun 6, 1998·The Journal of Biological Chemistry·G KrapivinskyD E Clapham
Aug 22, 2007·Journal of Cellular Physiology·Sharmistha Pal, Saïd Sif
Dec 14, 1999·The Journal of Biological Chemistry·P YangS Marcus
Nov 13, 2002·The Journal of Biological Chemistry·Timothy Y HuangDallan Young
Jan 6, 2005·The Biochemical Journal·Edward Wheatley, Katrin Rittinger
Dec 22, 1998·Molecular and Cellular Biology·H Tu, M Wigler
May 18, 1999·Molecular and Cellular Biology·W T PuD E Clapham
Apr 25, 2000·The Journal of Biological Chemistry·W T PuD E Clapham

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