The highly efficient T7 RNA polymerase: A wonder macromolecule in biological realm

International Journal of Biological Macromolecules
Subhomoi Borkotoky, Ayaluru Murali

Abstract

The study of bacteriophage has always been of keen interest for biologists to understand the fundamentals of biology. Bacteriophage T7 was first isolated in 1945 and its first comprehensive genetic map of was published in 1969. Since then, it has gained immense attention of researchers and became a prime model system for experimental biologists. The major gene product of T7 phage, T7 RNA polymerase (T7RNAP), continues to attract researchers since a long time due to its high and specific processivity with a single subunit structure and its capability of transcribing a complete gene without additional proteins. Since the first review article in 1993 there has been around nine reviews on this polymerase till year 2009, most of which focussed on particular aspects of T7RNAP such as structure and function. However, this review encapsulates a broad view on T7RNAP, one of the simplest macromolecule catalyzing RNA synthesis, including recent updates on its applications, structure, activators and inhibitors. Thus this brief review bridges the huge gap on the recent updates on this polymerase and will help the biologists in their endeavours that include the use of T7RNAP.

Citations

Mar 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hannes FeyrerKatja Petzold
Sep 23, 2020·RNA·Luiz F M PassalacquaAndrej Lupták
Jan 19, 2021·Chemical Communications : Chem Comm·Jisu WooKi Soo Park
Mar 13, 2020·Molecular Therapy. Nucleic Acids·Neetu SinghSuryaprakash Sambhara
Apr 21, 2020·Current Pharmaceutical Biotechnology·Atieh Hashemi, Gilar Gorji-Bahri
Jul 16, 2021·Critical Reviews in Biochemistry and Molecular Biology·Jörn GlöklerMarcus Frohme

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