The histone acetyltransferase GCN5 and the transcriptional coactivator ADA2b affect leaf development and trichome morphogenesis in Arabidopsis

Planta
Jenna KotakAmy T Hark

Abstract

The histone acetyltransferase GCN5 and associated transcriptional coactivator ADA2b are required to couple endoreduplication and trichome branching. Mutation of ADA2b also disrupts the relationship between ploidy and leaf cell size. Dynamic chromatin structure has been established as a general mechanism by which gene function is temporally and spatially regulated, but specific chromatin modifier function is less well understood. To address this question, we have investigated the role of the histone acetyltransferase GCN5 and the associated coactivator ADA2b in developmental events in Arabidopsis thaliana. Arabidopsis plants with T-DNA insertions in GCN5 (also known as HAG1) or ADA2b (also known as PROPORZ1) display pleiotropic phenotypes including dwarfism and floral defects affecting fertility. We undertook a detailed characterization of gcn5 and ada2b phenotypic effects in rosette leaves and trichomes to establish a role for epigenetic control in these developmental processes. ADA2b and GCN5 play specific roles in leaf tissue, affecting cell growth and division in rosette leaves often in complex and even opposite directions. Leaves of gcn5 plants display overall reduced ploidy levels, while ada2b-1 leaves show increased ploid...Continue Reading

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Citations

Oct 28, 2019·The New Phytologist·Robert Schuurink, Alain Tissier
Aug 4, 2020·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Klaus D GrasserFredy Barneche
Feb 28, 2021·Cellular and Molecular Life Sciences : CMLS·Verandra KumarManoj Prasad
May 1, 2021·Cells·Stylianos PouliosKonstantinos E Vlachonasios

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Methods Mentioned

BETA
acetylation
FCS
PCR
flow cytometry
Light microscopy
Scanning electron microscopy
ChIP-chip

Software Mentioned

Image J
Motic Images Plus
ImageJ

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