The histone acetyltransferase PCAF associates with actin and hnRNP U for RNA polymerase II transcription.

Molecular and Cellular Biology
Ales ObrdlikPiergiorgio Percipalle

Abstract

Actin is a key regulator of RNA polymerase (pol) II transcription. In complex with specific hnRNPs, it has been proposed that actin functions to recruit pol II coactivators during the elongation of nascent transcripts. Here, we show by affinity chromatography, protein-protein interaction assays, and biochemical fractionation of nuclear extracts that the histone acetyltransferase (HAT) PCAF associates with actin and hnRNP U. PCAF and the nuclear actin-associated HAT activity detected in the DNase I-bound protein fraction could be released by disruption of the actin-hnRNP U complex. In addition, actin, hnRNP U, and PCAF were found to be associated with the Ser2/5- and Ser2-phosphorylated pol II carboxy-terminal domain construct. Chromatin and RNA immunoprecipitation assays demonstrated that actin, hnRNP U, and PCAF are present at the promoters and coding regions of constitutively expressed pol II genes and that they are associated with ribonucleoprotein complexes. Finally, disruption of the actin-hnRNP U interaction repressed bromouridine triphosphate incorporation in living cells, suggesting that actin and hnRNP U cooperate with PCAF in the regulation of pol II transcription elongation.

References

Jun 5, 1998·Nucleic Acids Research·E R SmithC D Allis
Jul 23, 1998·Cell·V V OgryzkoY Nakatani
Sep 3, 1998·The Journal of Biological Chemistry·E MartinezR G Roeder
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·A KrummM Groudine
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·D E Sterner, S L Berger
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·T YamauchiY Nakatani
Aug 9, 2001·Journal of Molecular Biology·R Marmorstein
Apr 9, 2002·Nucleic Acids Research·Piergiorgio PercipalleBertil Daneholt
Jun 5, 2002·Annual Review of Biochemistry·Ivan A OlaveGerald R Crabtree
May 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Piergiorgio PercipalleNeus Visa
Feb 26, 2004·Experimental Cell Research·Nathalie Fomproix, Piergiorgio Percipalle
May 20, 2004·Molecular Cell·Christopher GilbertJesper Q Svejstrup
Oct 19, 2004·Genes & Development·Robert J SimsDanny Reinberg
Oct 27, 2004·Nature Cell Biology·Wilma A HofmannPrimal de Lanerolle
Feb 16, 2005·Nature Structural & Molecular Biology·Alexander KukalevPiergiorgio Percipalle
Jun 21, 2005·Genes & Development·Anton MeinhartPatrick Cramer
Nov 22, 2005·Journal of Structural Biology·C-A SchoenenbergerB M Jockusch
Mar 22, 2006·The Journal of Cell Biology·Piergiorgio Percipalle, Neus Visa
Apr 1, 2006·Current Opinion in Cell Biology·Piergiorgio Percipalle, Ann-Kristin Ostlund Farrants
Apr 25, 2006·Trends in Genetics : TIG·Jane Mellor
Jul 6, 2006·Nature Cell Biology·Erwann Vieu, Nouria Hernandez
Jan 16, 2007·The Journal of Biological Chemistry·Youngdong YooJun-Lin Guan
Feb 27, 2007·Cell·Bing LiJerry L Workman

❮ Previous
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Citations

Jan 1, 2013·Cellular and Molecular Life Sciences : CMLS·Kei Miyamoto, J B Gurdon
Nov 4, 2011·Nature Cell Biology·Primal de Lanerolle, Leonid Serebryannyy
Oct 4, 2013·Nature Reviews. Molecular Cell Biology·Robert Grosse, Maria K Vartiainen
Feb 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Joseph DopieMaria K Vartiainen
Mar 27, 2012·Molecular & Cellular Proteomics : MCP·Davina TondeleirChristophe Ampe
Oct 9, 2009·Molecular & Cellular Proteomics : MCP·Yun W LamDavid A Matthews
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Neus Visa, Piergiorgio Percipalle
Nov 10, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·George Fromm, Michael Bulger
Apr 7, 2010·Annual Review of Biochemistry·Luke A SelthJesper Q Svejstrup
Jan 2, 2013·Journal of Cell Science·Primal de Lanerolle
Nov 16, 2013·Nucleic Acids Research·Dilshad H KhanJames R Davie
Nov 20, 2014·Nature Communications·Pavan Kumar PuvvulaOliver Bischof
Feb 12, 2013·The Journal of Biological Chemistry·Ngoc T VuCharles E Chalfant
Mar 21, 2009·Cellular and Molecular Life Sciences : CMLS·Piergiorgio Percipalle
Feb 6, 2016·Histochemistry and Cell Biology·Ildikó KristóPéter Vilmos
Sep 10, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·James R DavieGenevieve P Delcuve
Jul 18, 2014·Nucleic Acids Research·Sébastien BrittonPatrick Calsou
Nov 8, 2015·The International Journal of Biochemistry & Cell Biology·Yinfang WangPeng Zhang
Apr 11, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Manuela Basso, Rajiv R Ratan
Aug 5, 2015·Histochemistry and Cell Biology·Marta Migocka-PatrzałekMaria Malicka-Błaszkiewicz
Jun 30, 2009·Journal of Cellular Biochemistry·Michael J ZeitzRonald Berezney
Jul 2, 2010·Cytoskeleton·Kari-Pekka Skarp, Maria K Vartiainen
Jun 12, 2013·Cytoskeleton·Kari-Pekka SkarpMaria K Vartiainen
Jul 18, 2009·Cell Motility and the Cytoskeleton·Andrew J Lindsay, Mary W McCaffrey
Apr 5, 2016·Cell Proliferation·Xiuqing HuangJian Li
May 11, 2012·Nucleus·Emilia SöderbergNeus Visa
Jul 3, 2013·Biochimica Et Biophysica Acta·Hai-shan BiShu-han Sun
Dec 1, 2012·Current Opinion in Cell Biology·Jonathan R Terman, Anna Kashina
Mar 25, 2014·Seminars in Cell & Developmental Biology·Piergiorgio Percipalle
Jul 10, 2012·Transcription·Guillaume HuetMaria K Vartiainen
Mar 8, 2014·Small GTPases·Eeva Kaisa Rajakylä, Maria K Vartiainen
Nov 10, 2012·Nucleus·Piergiorgio Percipalle
May 27, 2015·PloS One·Bruno LemieuxBenoit Chabot
Feb 13, 2016·Journal of Cellular Physiology·Dilshad H KhanJames R Davie
Jun 28, 2016·Scientific Reports·Leonid A SerebryannyyPrimal de Lanerolle

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