The histone deacetylase Rpd3/Sin3/Ume6 complex represses an acetate-inducible isoform of VTH2 in fermenting budding yeast cells

FEBS Letters
Igor StuparevicMichael Primig

Abstract

The tripartite Rpd3/Sin3/Ume6 complex represses meiotic isoforms during mitosis. We asked if it also controls starvation-induced isoforms. We report that VTH1/VTH2 encode acetate-inducible isoforms with extended 5'-regions overlapping antisense long non-coding RNAs. Rpd3 and Ume6 repress the long isoform of VTH2 during fermentation. Cells metabolising glucose contain Vth2, while the protein is undetectable in acetate and during sporulation. VTH2 is a useful model locus to study mechanisms implicating promoter directionality, lncRNA transcription and post-transcriptional control of gene expression via 5'-UTRs. Since mammalian genes encode transcript isoforms and Rpd3 is conserved, our findings are relevant for gene expression in higher eukaryotes.

References

Sep 1, 1992·Molecular and Cellular Biology·R T Surosky, R E Esposito
Apr 1, 1994·Genes & Development·R StrichR E Esposito
May 1, 1994·Molecular and Cellular Biology·R T SuroskyR E Esposito
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·C M Steber, R E Esposito
May 17, 1996·The Journal of Biological Chemistry·V WestphalH B van den Hazel
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·N SukaM Grunstein
Dec 2, 2000·Nature Genetics·M PrimigR E Esposito
Jan 30, 2002·Gene·Irene T RombelStephen Albert Johnston
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Roy M WilliamsRochelle E Esposito
Nov 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Adam M DeutschbauerRonald W Davis
Jun 12, 2003·Nucleic Acids Research·Mitsuhiro ShimizuAaron P Mitchell
Dec 31, 2005·Nucleic Acids Research·V MatysE Wingender
Nov 18, 2006·Cell·Cintia F HongayGerald R Fink
Sep 25, 2007·Molecular Cell·Michael J MalloryRandy Strich
Oct 4, 2007·Annals of the New York Academy of Sciences·Frederic ChalmelMichael Primig
Feb 23, 2008·Nature Reviews. Molecular Cell Biology·Xiang-Jiao Yang, Edward Seto
May 3, 2008·Science·Ugrappa NagalakshmiMichael Snyder
Jan 27, 2009·Nature·Zhenyu XuLars M Steinmetz
Jun 12, 2009·Annual Review of Microbiology·Heather M HoodMatthew S Sachs
Mar 3, 2010·Genome Biology·Marina V GranovskaiaLars M Steinmetz
Nov 16, 2010·Current Opinion in Biotechnology·Ulrike MäderStéphane Aymerich
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Aurélie LardenoisMichael Primig
May 24, 2011·Trends in Genetics : TIG·Wu WeiLars M Steinmetz
Dec 24, 2011·Science·Gloria A BrarJonathan S Weissman
Apr 28, 2012·Cellular and Molecular Life Sciences : CMLS·Lucy W BarrettSteve D Wilton
Jun 5, 2012·Nucleic Acids Research·Panu ArtimoHeinz Stockinger
Aug 28, 2012·Molecular Cell·Thomas Beuchert KallehaugeTorben Heick Jensen
Sep 25, 2012·Molecular Cell·Rajani Kanth GudipatiDomenico Libri
Feb 8, 2013·G3 : Genes - Genomes - Genetics·Karl Waern, Michael Snyder
Apr 26, 2013·Nature·Vicent PelechanoLars M Steinmetz

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