The Histone Demethylase JMJD2A Modulates the Induction of Hypertrophy Markers in iPSC-Derived Cardiomyocytes

Frontiers in Genetics
Wendy Rosales, Fernando Lizcano

Abstract

The development of cardiovascular pathologies is partly attributed to epigenetic causes, including histone methylation, which appears to be an important marker in hearts that develop cardiac hypertrophy. Previous studies showed that the histone demethylase JMJD2A can regulate the hypertrophic process in murine cardiomyocytes. However, the influence of JMJD2A on cardiac hypertrophy in a human cardiomyocyte model is still poorly understood. In the present study, cardiomyocytes derived from human induced pluripotent stem cells (iPSCs) were used. Hypertrophy was induced by angiotensin II and endothelin-1 (ET-1), and transfections were performed to overexpress JMJD2A and for small interfering RNA (siRNA)-induced silencing of JMJD2A. Gene expression analyses were determined using RT-PCR and Western blot. The expression levels of B-type natriuretic peptide (BNP), natriuretic peptide A (ANP), and beta myosin heavy chain (β-MHC) were increased by nearly 2-10-fold with ET-1 compared with the control. However, a higher level of JMJD2A and UTX was detected, whereas the level of JMJD2C was lower. When cardiomyocytes were transiently transfected with JMJD2A, an increase close to 150% in BNP was observed, and this increase was greater after t...Continue Reading

References

Jun 2, 2005·The Journal of Biological Chemistry·Steven G GrayFernando Dangond
Apr 23, 2010·Journal of Visualized Experiments : JoVE·Angela C deAlmeidaXander H T Wehrens
Jun 4, 2011·Genetics and Molecular Biology·Fernando LizcanoDiana Vargas
Sep 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Junyi MaCraig T January
Jul 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·G Grant WelsteadRudolf Jaenisch
Jul 31, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Payal RanaYvonne Will
Aug 23, 2012·PloS One·Tepmanas Bupha-IntrPaul M L Janssen
Feb 26, 2013·The Journal of Clinical Investigation·Mathias HohlChristoph Maack
Jun 28, 2013·American Journal of Physiology. Cell Physiology·Michael FineDonald W Hilgemann
Jan 1, 2012·Pharmaceuticals·Fernando Lizcano, Jeison Garcia
Oct 25, 2014·F1000prime Reports·Zachary A Gurard-Levin, Geneviève Almouzni
Jun 6, 2016·Journal of Molecular and Cellular Cardiology·Ippei Shimizu, Tohru Minamino
Oct 11, 2016·BioMed Research International·Wendy RosalesFernando Lizcano
Sep 16, 2017·Circulation Research·William J McKennaGaetano Thiene

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Citations

Apr 6, 2019·Journal of Cellular and Molecular Medicine·Kaowen YanPeifeng Li
Apr 17, 2020·Heart Failure Reviews·Hao LeiDanyan Xu
Jun 20, 2020·Cellular and Molecular Life Sciences : CMLS·Speranza RubattuMaurizio Forte
May 12, 2020·Frontiers in Physiology·Sebastian MartewiczNicola Elvassore
Nov 22, 2020·International Journal of Molecular Sciences·Felipe ArroyaveFernando Lizcano

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Methods Mentioned

BETA
transfection
transfections

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