The histone methyltransferase Set7/9 promotes myoblast differentiation and myofibril assembly.

The Journal of Cell Biology
Yazhong TaoDa-Zhi Wang

Abstract

The molecular events that modulate chromatin structure during skeletal muscle differentiation are still poorly understood. We report in this paper that expression of the H3-K4 histone methyltransferase Set7 is increased when myoblasts differentiate into myotubes and is required for skeletal muscle development, expression of muscle contractile proteins, and myofibril assembly. Knockdown of Set7 or expression of a dominant-negative Set7 mutant impairs skeletal muscle differentiation, accompanied by a decrease in levels of histone monomethylation (H3-K4me1). Set7 directly interacts with MyoD to enhance expression of muscle differentiation genes. Expression of myocyte enhancer factor 2 and genes encoding contractile proteins is decreased in Set7 knockdown myocytes. Furthermore, we demonstrate that Set7 also activates muscle gene expression by precluding Suv39h1-mediated H3-K9 methylation on the promoters of myogenic differentiation genes. Together, our experiments define a biological function for Set7 in muscle differentiation and provide a molecular mechanism by which Set7 modulates myogenic transcription factors during muscle differentiation.

References

Nov 15, 1985·Science·H M BlauC Webster
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H WeintraubA D Miller
Aug 1, 1996·Current Opinion in Genetics & Development·J D Molkentin, E N Olson
Nov 1, 1996·Molecular and Cellular Biology·M SoulezD Tuil
Sep 26, 1997·Genes & Development·C S BlagdenS M Hughes
Oct 31, 1998·Current Opinion in Genetics & Development·H H Arnold, B Winter
Aug 17, 2000·The Journal of Biological Chemistry·A PolesskayaA Harel-Bellan
Sep 5, 2001·Current Opinion in Genetics & Development·T A McKinseyE N Olson
Mar 15, 2002·Current Opinion in Genetics & Development·Tony Kouzarides
Feb 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Asoke Mal, Marian L Harter
May 22, 2003·Cell·Valerie HorsleyGrace K Pavlath
Oct 31, 2003·Trends in Genetics : TIG·Robert J SimsDanny Reinberg
Apr 22, 2004·Molecular Cell·Antigone KouskoutiIannis Talianidis
Nov 3, 2004·Genes & Development·Giuseppina CarettiVittorio Sartorelli
Aug 2, 2005·Current Opinion in Genetics & Development·Vittorio Sartorelli, Giuseppina Caretti
Aug 16, 2005·Seminars in Cell & Developmental Biology·Charlotte A Berkes, Stephen J Tapscott
Sep 1, 2005·Seminars in Cell & Developmental Biology·Sonia Vanina Forcales, Pier Lorenzo Puri
Sep 6, 2005·The Journal of Biological Chemistry·Joshua FrancisRaghavendra G Mirmira
Nov 11, 2005·Development·Katherine R DohertyElizabeth M McNally
Oct 31, 2006·Current Opinion in Structural Biology·Jean-François Couture, Raymond C Trievel
Mar 8, 2007·Nature Reviews. Molecular Cell Biology·Robert J Klose, Yi Zhang
Jun 1, 2007·Development·Yaniv Hinits, Simon M Hughes
Nov 27, 2007·Nature Reviews. Molecular Cell Biology·Alexander J RuthenburgC David Allis
Jan 29, 2008·Experimental Biology and Medicine·Thomas E CallisDa-Zhi Wang
Feb 19, 2008·Molecular Cell·Julia K KurashFrançois Gaudet

❮ Previous
Next ❯

Citations

Dec 20, 2012·Genes & Development·Roy BlumBrian D Dynlacht
Jul 20, 2012·Comparative and Functional Genomics·Herve Faralli, F Jeffrey Dilworth
Nov 13, 2013·Cellular and Molecular Life Sciences : CMLS·Nelsa L Estrella, Francisco J Naya
May 31, 2014·Cellular and Molecular Life Sciences : CMLS·Samuel T KeatingAssam El-Osta
Feb 18, 2015·Journal of Molecular Biology·Alexandre Blais
Sep 25, 2014·The FEBS Journal·Jessica SegalésPura Muñoz-Cánoves
Oct 21, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ok Hyun ChoAnthony N Imbalzano
Sep 4, 2015·Journal of Biosciences·Fathima Athar, Veena K Parnaik
Jul 10, 2012·Transcription·Narendra Bharathy, Reshma Taneja
Jul 25, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Roy Blum, Brian D Dynlacht
Mar 13, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Samuel T Keating, Assam El-Osta
Mar 12, 2013·Molecular Cell·Eliezer Calo, Joanna Wysocka
Jul 16, 2013·Developmental Cell·Menno J OudhoffColby Zaph
Dec 24, 2014·Biochemical and Biophysical Research Communications·Jang Hyun ChoiHansol Lee
Nov 20, 2014·Frontiers in Genetics·Kyohei OyamaW Robb MacLellan
Nov 2, 2016·Nucleic Acids Research·Natasha K TuanoAssam El-Osta
Sep 15, 2016·Frontiers in Cell and Developmental Biology·Jessica SegalésPura Muñoz-Cánoves
Nov 4, 2017·Epigenetics : Official Journal of the DNA Methylation Society·Theodore VougiouklakisVassiliki Saloura
Sep 1, 2016·Development·Julia KofentFrancesca M Spagnoli
Mar 16, 2019·The Journal of Biological Chemistry·Natarajan V BhanuBenjamin A Garcia
Jan 17, 2020·Transcription·Jie YaoMin Wu
Feb 26, 2020·The FEBS Journal·Arnab Nayak, Mamta Amrute-Nayak
Mar 22, 2016·Biomedical Reports·Jifu LiHongjuan Cui
Sep 14, 2017·International Journal of Molecular Medicine·Xin YiDing-Sheng Jiang
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Vincenzo Cavalieri
Dec 4, 2016·Non-coding RNA·Nazanin KarimniaAssam El-Osta
Jul 18, 2018·Signal Transduction and Targeted Therapy·Inês de Albuquerque Almeida Batista, Luisa Alejandra Helguero

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
transfection
pull-down
coimmunoprecipitation
co-IP
PCR
ChIP
electrophoresis
transmission electron microscopy
Methyltransferase

Software Mentioned

Photoshop
SPOT
BLASTp

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.