The histone variant H2A.Z is an important regulator of enhancer activity

Nucleic Acids Research
Mylène BrunelleNicolas Gévry

Abstract

Gene regulatory programs in different cell types are largely defined through cell-specific enhancers activity. The histone variant H2A.Z has been shown to play important roles in transcription mainly by controlling proximal promoters, but its effect on enhancer functions remains unclear. Here, we demonstrate by genome-wide approaches that H2A.Z is present at a subset of active enhancers bound by the estrogen receptor alpha (ERα). We also determine that H2A.Z does not influence the local nucleosome positioning around ERα enhancers using ChIP sequencing at nucleosomal resolution and unsupervised pattern discovery. We further highlight that H2A.Z-enriched enhancers are associated with chromatin accessibility, H3K122ac enrichment and hypomethylated DNA. Moreover, upon estrogen stimulation, the enhancers occupied by H2A.Z produce enhancer RNAs (eRNAs), and recruit RNA polymerase II as well as RAD21, a member of the cohesin complex involved in chromatin interactions between enhancers and promoters. Importantly, their recruitment and eRNAs production are abolished by H2A.Z depletion, thereby revealing a novel functional link between H2A.Z occupancy and enhancer activity. Taken together, our findings suggest that H2A.Z acts as an impor...Continue Reading

References

Jan 1, 1988·Annual Review of Biochemistry·D S Gross, W T Garrard
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Jul 20, 2004·Nature Structural & Molecular Biology·Gu Li, Jonathan Widom
Dec 8, 2004·Nature Structural & Molecular Biology·Gu LiJonathan Widom
Oct 3, 2006·Nature Genetics·Jason S CarrollMyles Brown
Mar 10, 2007·Science·Yoshiko MitoSteven Henikoff
Jun 19, 2007·Genes & Development·Chunyuan Jin, Gary Felsenfeld
Aug 3, 2007·Genes & Development·Nicolas GévryLuc Gaudreau
Mar 7, 2008·Nature·Raphaël MétivierGilles Salbert
Mar 11, 2008·Cell·Dustin E SchonesKeji Zhao
Mar 18, 2008·Molecular Cell·Sam JohnGordon L Hager
May 20, 2008·Journal of Molecular Biology·Michael G PoirierJonathan Widom
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Sep 26, 2008·Nature·Daniel ZilbermanSteven Henikoff
Dec 18, 2008·Genome Research·Steven HenikoffKami Ahmad
Feb 13, 2009·Nature·Axel ViselLen A Pennacchio
Feb 28, 2009·Genome Research·Michael Y TolstorukovPeter J Park
Apr 21, 2009·Methods in Molecular Biology·Amy SvotelisLuc Gaudreau
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 12, 2009·Genes & Development·Nicolas GévryLuc Gaudreau
Nov 6, 2009·Nature·Melissa J FullwoodYijun Ruan
Dec 22, 2009·Cell Cycle·Amy SvotelisLuc Gaudreau
Feb 18, 2010·PloS One·Desiree TilloTimothy R Hughes
Mar 9, 2010·Nature Genetics·Housheng Hansen HeX Shirley Liu
Mar 12, 2010·Genome Research·Dominic SchmidtDuncan T Odom
Apr 16, 2010·Nature·Tae-Kyung KimMichael E Greenberg
May 4, 2010·Nature Biotechnology·Cory Y McLeanGill Bejerano
May 21, 2010·PLoS Biology·Francesca De SantaGioacchino Natoli
Aug 3, 2010·PloS One·Sébastien RodrigueSallie W Chisholm
Aug 17, 2010·Genome Research·Melissa L ConerlySteven Henikoff
Aug 20, 2010·Nature·Michael H KageyRichard A Young
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Menno P CreyghtonRudolf Jaenisch

❮ Previous
Next ❯

Citations

Dec 29, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alexia Martínez de Paz, Juan Ausió
Jul 1, 2016·Open Biology·Naoki HorikoshiHitoshi Kurumizaka
Jan 9, 2019·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Tom MossMarianne Sabourin-Felix
Mar 6, 2019·Scientific Data·Andrea A PerreaultBryan J Venters
Nov 5, 2019·Genes & Development·Bjoern GaertnerMaike Sander
Jun 16, 2019·Epigenetics & Chromatin·Benedetto Daniele GiaimoTilman Borggrefe
Apr 30, 2017·Transcription·Gregory Segala, Didier Picard
Nov 9, 2017·Nature Communications·Fátima Valdés-MoraSusan J Clark
Feb 24, 2019·Genome Research·Kasper D RasmussenKristian Helin
Jan 30, 2020·Frontiers in Cell and Developmental Biology·Preston R ArnoldXian C Li
Nov 11, 2020·Cells·Juliette FerrandSophie E Polo
Mar 27, 2021·The Journal of Physical Chemistry. B·Daniel EspirituAnna R Panchenko
Mar 28, 2021·Journal of Cell Science·Paul B Talbert, Steven Henikoff

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE57436

Methods Mentioned

BETA
ChIP-chip
immunoprecipitation
ChIP-seq
ChIP
MNase-seq
MNase-sequencing
acetylation

Software Mentioned

GREAT
CAGT
MACS
BWA ( Aligner )
CAGT ( Clustered AGgregation Tool )
Wiggler
seqMINER

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.