The hitchhiker's guide to Xenopus genetics.

Genesis : the Journal of Genetics and Development
Anita Abu-DayaLyle B Zimmerman

Abstract

A decade after the human genome sequence, most vertebrate gene functions remain poorly understood, limiting benefits to human health from rapidly advancing genomic technologies. Systematic in vivo functional analysis is ideally suited to the experimentally accessible Xenopus embryo, which combines embryological accessibility with a broad range of transgenic, biochemical, and gain-of-function assays. The diploid X. tropicalis adds loss-of-function genetics and enhanced genomics to this repertoire. In the last decade, diverse phenotypes have been recovered from genetic screens, mutations have been cloned, and reverse genetics in the form of TILLING and targeted gene editing have been established. Simple haploid genetics and gynogenesis and the very large number of embryos produced streamline screening and mapping. Improved genomic resources and the revolution in high-throughput sequencing are transforming mutation cloning and reverse genetic approaches. The combination of loss-of-function mutant backgrounds with the diverse array of conventional Xenopus assays offers a uniquely flexible platform for analysis of gene function in vertebrate development.

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Citations

May 30, 2013·BMC Genomics·Eva SeifertovaVladimir Krylov
Mar 1, 2014·Advanced Drug Delivery Reviews·Stefan M SchmittAndré W Brändli
Dec 5, 2013·Biological Reviews of the Cambridge Philosophical Society·Iain S HaslamRalf Paus
Oct 15, 2013·Genesis : the Journal of Genetics and Development·Takuya NakayamaRobert M Grainger
Jan 18, 2017·Genesis : the Journal of Genetics and Development·Thomas NaertKris Vleminckx
Jan 31, 2012·Genesis : the Journal of Genetics and Development·Mustafa K Khokha

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