The H,K-ATPase beta subunit as a model to study the role of N-glycosylation in membrane trafficking and apical sorting

The Journal of Biological Chemistry
Olga VaginGeorge Sachs

Abstract

The role of N-glycosylation in trafficking of an apical membrane protein, the gastric H,K-ATPase beta subunit linked to yellow fluorescent protein, was analyzed in polarized LLC-PK1 cells by confocal microscopy and surface-specific biotinylation. Deletion of the N-glycosylation sites at N1, N3, N5, and N7 but not at N2, N4, and N6 significantly slowed endoplasmic reticulum-to-Golgi trafficking, impaired apical sorting, and enhanced endocytosis from the apical membrane, resulting in decreased apical expression. Golgi mannosidase inhibition to prevent carbohydrate chain branching and elongation resulted in faster internalization and degradation of the beta subunit, indicating that terminal glycosylation is important for stabilization of the protein in the apical membrane and protection of internalized protein from targeting to the degradation pathway. The decrease in the apical content of the beta subunit was less with mannosidase inhibition compared with that found in the N1, N3, N5, and N7 site mutants, suggesting that the core region sugars are more important than the terminal sugars for apical sorting.

References

Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M A ReubenG Sachs
Nov 2, 1995·Nature·P ScheiffeleK Simons
Aug 1, 1994·Current Opinion in Cell Biology·K Matter, I Mellman
Apr 1, 1993·The Journal of Cell Biology·C J Gottardi, M J Caplan
Apr 1, 1996·The Journal of Cell Biology·T YoshimoriK Simons
Aug 1, 1997·Current Opinion in Cell Biology·L M Traub, S Kornfeld
Dec 4, 1998·Seminars in Cell & Developmental Biology·E Ikonen, K Simons
Jan 29, 1999·Physiological Reviews·C YeamanW J Nelson
Jul 17, 1999·Trends in Cell Biology·E Rodriguez-Boulan, A Gonzalez
Feb 15, 2001·Nature Cell Biology·P KellerK Simons
Mar 28, 2001·Science·A Helenius, M Aebi
Jan 5, 2002·Journal of Bioenergetics and Biomembranes·K Geering
Mar 23, 2002·The Journal of Biological Chemistry·Tohru KimuraShinji Asano
May 30, 2003·American Journal of Physiology. Cell Physiology·O VaginG Sachs
Jan 9, 2004·The New England Journal of Medicine·Patricia D Wilson
Apr 2, 2004·Nature Cell Biology·W James Nelson, Enrique Rodriguez-Boulan

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Citations

Jun 10, 2008·Pflügers Archiv : European journal of physiology·Jai Moo ShinGeorge Sachs
Apr 29, 2006·Digestive Diseases and Sciences·Jai Moo Shin, George Sachs
Nov 14, 2008·Current Gastroenterology Reports·Jai Moo Shin, George Sachs
Mar 5, 2010·Biochemistry·Elmira TokhtaevaOlga Vagin
Sep 14, 2010·The Journal of Biological Chemistry·Nicole L GlossonAmy W Hudson
Dec 20, 2012·The Journal of Biological Chemistry·Alexandra MantIta O'Kelly
Feb 13, 2009·American Journal of Physiology. Cell Physiology·Veedamali S SubramanianHamid M Said
Oct 31, 2008·American Journal of Physiology. Renal Physiology·Olga VaginGeorge Sachs
Nov 13, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maria MastrodonatoDomenico Ferri
Jul 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Seung-Kuy ChaChou-Long Huang
Sep 1, 2006·Expert Opinion on Drug Discovery·Peter P Mager, Peter Illes
Aug 23, 2016·Nature Chemical Biology·Shinya HirayamaKazuya Kikuchi
Aug 11, 2006·The Journal of Biological Chemistry·Tsutomu NakagawaEiji Miyoshi
Apr 13, 2013·International Journal of Molecular Sciences·Vincent A van der MarkCoen C Paulusma
Jul 25, 2008·Proteomics·Anne-Sophie Vercoutter-EdouartJean-Claude Michalski
Dec 13, 2005·American Journal of Physiology. Cell Physiology·Beth A PotterOra A Weisz
Dec 22, 2006·Traffic·Fedor Berditchevski, Elena Odintsova
Nov 17, 2009·Biology of the Cell·Monica CarmosinoMaria Svelto
Jul 6, 2014·Physiology·Pirjo M Apaja, Gergely L Lukacs
Mar 9, 2007·American Journal of Physiology. Renal Physiology·Melissa D LaugheryJack H Kaplan
Nov 2, 2019·Physiological Reviews·Amy C EngevikJames R Goldenring
Oct 15, 2008·The Journal of Biological Chemistry·Gouri BaldwinFedor Berditchevski
May 11, 2010·FEBS Letters·Coen C Paulusma, Ronald P J Oude Elferink
Aug 16, 2019·Cells·Qiongyu LiCarlito B Lebrilla
Jun 11, 2019·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Takuto FujiiHideki Sakai
Oct 1, 2021·Nature Communications·Kazuhiro AbeAtsunori Oshima
May 17, 2011·Current Opinion in Cell Biology·Tsukasa OkiyonedaGergely L Lukacs

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